补阳还五汤对心肌梗死后大鼠心肌微血管密度及血管内皮细胞生长因子-C表达的影响

目的观察补阳还五汤对大鼠心肌梗死后左室非梗死区心肌微血管新生的影响。方法雄性Wistar大鼠,结扎左冠状动脉主干造成心肌梗死模型,将造模成功的大鼠随机分为模型对照组及补阳还五汤低、中、高剂量组,加上假手术组,共5组,每组12只。补阳还五汤低、中、高剂量组分别按9.25、18.5、37 g/（kg.d）给予补阳还五汤水煎剂灌胃,假手术组、模型组给予0.2 mL/10 g生理盐水灌胃。连续给药12周后处死大鼠。免疫组化法检测心肌Ⅷ因子的表达,Westren blot及免疫组化法检测血管内皮细胞生长因子-C（VEGF-C）的表达。结果假手术组和不同剂量补阳还五汤组血管密度和VEGF-C的表达明显高于模型组（P〈0.001）;补阳还五汤中剂量组VEGF-C的表达与假手术组相当（P〉0.05）,补阳还五汤高剂量组VEGF-C表达高于假手术组（P〈0.01）。结论补阳还五汤可以促进心肌梗死后大鼠左室非梗死区血管新生,并且呈剂量依赖性,补阳还五汤促血管新生作用可能与升高VEGF-C表达有关。

Influence of Buyanghuanwu Decoction on Myocardium Microvessel Density and Vascular Endothelial Growth Factor C Expression after Myocardial Infarction in Rats

Objective To investigate the effects of Buyanghuanwu decoction on myocardium microvessel density and vascular endothelial growth factor C（VEGF-C） expression in the non-infarction regions of left ventricle in rats after acute myocardial infarction.Methods Selecting male Wistar SPF rats,ligating the left main coronary artery to make acute myocardial infarction model.After successful modeling,rats were divided into model group,sham group,Buyanghuanwu decoction low dose,medium dose and high dose group,12 rats in each group.Buyanghuanwu decoction low dose,middle dose and high dose group were respectively given 9.25,18.5,37 g/（kg？d） decoction by intragastric administration,model group and sham group were given 0.2 mL/（10 g？d） normal saline,with successive administration for 12 weeks.Then the rats were killed.The myocardium Ⅷ factor was detected by immunohistochemistry.VEGF-C was detected by Westren blot and immunohistochemistry.Results The blood vessel density and VEGF-C expression of sham group and Buyanghuanwu decoction groups were obviously higher than sham group（P0.001）.Vessel density and VEGF-C expression of high dose group were higher than model group（P0.01）,and that of middle dose group had no difference with sham group（P0.05）.Conclusions Buyanghuanwu decoction could promote blood vessel neogenesis in the non-infarction regions of left ventricle in rats after acute myocardial infarction with dose-effect relationship,it maybe concerned with the effect of promoting the VEGF-C expression.