| Rapid Isolation of Large Amount of Plasma VLDL and LDL by a Two Step Ultracentrifugation |
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| 引用本文： | 王淳本,冯友梅,宗义强,邓耀祖,冯宗忱. Rapid Isolation of Large Amount of Plasma VLDL and LDL by a Two Step Ultracentrifugation[J]. 华中科技大学学报(医学英德文版), 1995, 15(4): 198-200. DOI: 10.1007/BF02887943 |
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| 作者姓名： | 王淳本 冯友梅 宗义强 邓耀祖 冯宗忱 |
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| 作者单位： | Department of Biochemistry，School of Basic Medical Sciences，Tongji Medical University，Wuhan 430030 |
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| 摘 要： | ＲａｐｉｄＩｓｏｌａｔｉｏｎｏｆＬａｒｇｅＡｍｏｕｎｔｏｆＰｌａｓｍａＶＬＤＬａｎｄＬＤＬｂｙａＴｗｏＳｔｅｐＵｌｔｒａｃｅｎｔｒｉｆｕｇａｔｉｏｎＷＡＮＧＣｈｕｎ－ｂｅｎ（王淳本）；ＦＥＮＧＹｏｕ－ｍｅｉ（冯友梅）；ＺＯＮＧＹｉ－ｑｉａｎｇ（宗义强...
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| 收稿时间： | 1995-02-28 |
Rapid isolation of large amount of plasma VLDL and LDL by a two step ultracentrifugation |
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| Wang Chun-ben,Feng You-mei,Zong Yi-qiang,Deng Yau-zu,Feng Zong-chen. Rapid isolation of large amount of plasma VLDL and LDL by a two step ultracentrifugation[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 1995, 15(4): 198-200. DOI: 10.1007/BF02887943 |
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| Authors: | Wang Chun-ben Feng You-mei Zong Yi-qiang Deng Yau-zu Feng Zong-chen |
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| Affiliation: | (1) Department of Biochemistry, School of Basic Medical Sciences, Tongji Medical University, 430030 Wuhan |
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| Abstract: | A rapid method to obtain large amount of VLDL and LDL by ultracentrifugation is described. The mixture of VLDL and LDL was isolated and concentrated from plasma by an ultracentrifugation at 265 000 g for 2h. VLDL and LDL were separated and purified by a further ultracentrifugation at 265 000g for 3h.This method combines the advantages of both sequential flotation ultracentri-fugation and density gradient ultracentrifugation. It can process a large volume of plasma in a short time. The purity of isolated VLDL and LDL was confirmed by the lipoprotein electrophoresis on agarose gel and PAGE and by the apolipoprotein electrophoresis on SDS-PAGE. This rapid.economical method is of great value in practical application. |
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| Keywords: | ultracentrifugation very low density lipoprotein (VLDL) low density lipoprotein (LDL) |
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