自然流产绒毛与蜕膜组织中细胞凋亡的研究

目的研究细胞凋亡在自然流产患者绒毛和蜕膜组织中的表达及其在自然流产中的作用。方法应用TdT介导的dUTP缺口原位末端标记技术（terminal deoxvnucleotidvl transferase-mediated dUTP-biotin nick endinglabeling，TUN-EL）检测40例早孕自然流产病例（研究组）和40例早孕人工流产病例（对照组）绒毛和蜕膜组织中细胞凋亡情况。结果凋亡指数在自然流产绒毛细胞滋养层细胞、合体滋养层细胞和蜕膜中分别为（24．54±3．21）％、（40．25±5．27）％和（56．14±4．56）％，早孕人工流产分别为（10．31±1．25）％、（15．65±5．45）％和（10．54±1．82）％，两组间差异有高度显著性（P〈0．01）。结论自然流产患者绒毛和蜕膜组织中细胞凋亡显著增加，其在自然流产的发生发展中起一定的作用。

Study of apoptosis in chorion villi and decidua in spontaneous abortion

Objective To study the expression of apoptosis in chorion villi and decidua and its effects on spontaneous abortion. Methods Chorion villi and decidua of first-trimester were obtained from 40 surgical aspiration and 40 spontaneous abortion cases. Apoptosis was detected by using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick ending labeling （TUNEL）. Results In spontaneous abortion, the apoptosis indexes （AI） of cytotrophoblasts, syncytotrophoblasts and decidua were 24.54±3.21, 40.25±5.27 and 56.14±4.56, while in normal pregnancies they were 10.31±1.25, 15.65±5.45 and 10.54±1.82. The apoptosis indexes were significantly increased in spontaneous abortion by comparision with their counterparts in surgical aspiration （p 〈0.01）. Conclusion The apoptosis indexes increase in spontaneous abortion. Apoptosis may play crucial roles in the development of spontaneous abortion.