脑胶质瘤EZH2基因的表达分析

目的探讨脑胶质瘤果蝇zeste基因增强子同源物2（EZH2）蛋白的表达变化。方法选取2010年5月至2011年12月手术切除的胶质瘤组织标本83例,其中2008年WHO分级Ⅰ级15例,Ⅱ级20,Ⅲ级26例,Ⅳ级22例;术前均未接受放疗或化疗。另外,收集30例颅脑损伤内减压手术切除的无肿瘤脑组织作为对照。采用实时荧光定量PCR法检测EZH2 m RNA表达。根据EZH2 m RNA表达水平分为高表达组（EZH2 m RNA≥0.3,48例）和低表达组（EZH2 m RNA〈0.3,35例）,分析两组术后生存期。结果胶质瘤组织EZH2 m RNA表达水平明显高于对照组（P〈0.01）,高级别胶质瘤（WHOⅢ、Ⅳ级）组织EZH2 m RNA表达水平明显高于低级别胶质瘤（WHOⅠ、Ⅱ级）组织（P〈0.01）。低表达组患者术后生存期较高表达组显著延长（P〈0.01）。结论脑胶质瘤组织EZH2 m RNA呈高表达,且其表达水平与病理分级具有相关性。

Analysis of expression of Enhancer of Zeste Homolog 2 in human gliomas

Objective To explore the expression and clinical meanings of enhancer of zeste homolog 2 （EZH2） protein in human gliomas. Methods The expressions of EZH2 mRNA were determined by a real-time PCR in 83 samples of human gliomas, of which, 35 were derived from the low （ Ⅰ~Ⅱ ） WHO grade and 48 from high （ Ⅲ -Ⅳ） WHO grade, and 30 samples of normal brain tissues. Results The Level （0.583 ±0.036） of EZH2 mRNA expression was significantly higher in WHO grades Ⅲ ~ Ⅳ gliomas than that （0.432±0.04） WHO grades Ⅰ ~ Ⅱ gliomas （P〈0.01）, which was significantly higher than that （0.2236±0.03） in the normal brain tissues （P〈0.001）. The expression level of EZH2 was positively related to the WHO grade of gliomas （P〈0.01）. Conclusion It is suggested that the upregulation of EZH2 mRNA expression in the gliomas tissues may be play an important role in the pathogenesis and development of the gliomas.