趋化因子受体CXCR4在鼻咽癌中的表达及意义

目的探讨趋化因子受体CXCR4在人鼻咽癌中的表达及临床意义。方法采用Real-timeRT-PCR法检测鼻咽癌细胞株中CXCR4mRNA的表达，应用免疫组织化学法并结合组织阵列检测正常鼻咽组织、鼻咽癌和鼻咽癌淋巴结转移石蜡组织标本中CXCR4蛋白的表达情况。结果在7种鼻咽癌细胞株中，CXCR4基因在高成瘤、高转移潜能的5-8F细胞中表达水平最高，在无转移能力的610B细胞中表达最低。CXCR4蛋白在正常鼻咽组织、鼻咽癌和鼻咽癌淋巴结转移组织中表达差异具有统计学意义（P=0．002）；鼻咽癌组织中CXCR4表达高于正常鼻咽组（P=0．029）；伴发转移的鼻咽癌组织比未发生转移的鼻咽癌组织CXCR4表达增强，差异具有统计学意义（P=0．008）；淋巴结转移癌组织CX-CR4表达比鼻咽癌组织高（P=0．013）。结论本研究提示CXCR4表达与鼻咽癌转移存在密切关系，CXCR4表达可作为鼻咽癌转移过程中一个有价值的指标。

Expression of Chemokine Receptor CXCR4 in Nasopharyngeal Carcinomas and Its Clinical Significance

Objective To investigate the relationship of the expressions of chemokine receptor CXCR4 with metastasis and its clinical significance in human nasopharyngeal carcinoma(NPC).Methods The expression of CXCR4 mRNA in seven NPC cell lines was detected by Real-time reverse polymerase chain reaction(RT-PCR),and immuno-histochemistry was used to detect CXCR4 expression in a tissue array composed with nasopharynx epithelium(NE),NPC and lymphatic metastasis of NPC.Results CXCR4 mRNA was highly expressed in 5-8F cell line,which tumorigenic and high metastatic ability,lowly expressed in 6-10B cell line with tumorigenic,lacking metastatic ability,and moderately in C666-1,CNE-1, CEN-2,HONE-1 and HNE-1.The expression of CXCR4 protein was significantly differential among the NE,NPC and lymphatic metastasis of NPC(P=0.002).CXCR4 was higher expressed in carcinoma compared with NE(P=0.029).The CXCR4 expression in NPC with metastasis was higher than that in NPC without metastasis(P=0.008).Furthermore,CXCR4 expression was significantly stronger in lymphatic metastasis carcinoma tissues comparison with NPC(P=0.013).Conclusion CXCR4 plays an important role in invasion and metastasis of NPC and may be a useful indictor of the tumor metastasis in NPC.