药用甘薯西蒙1号胚状体的诱导及植株再生

甘薯西蒙１号的叶片在ＭＳ＋２,４－Ｄ０．５ｍｇ／Ｌ＋ＫＴ０．５ｍｇ／Ｌ＋ＡＢＡ５ｍｇ／Ｌ的培养基上易于诱导出愈伤组织,来源于叶片的愈伤分为胚性和非胚性,ＮＥＣ的增殖速度大于ＥＣ,继代培养时应及时将两种愈伤组织剥离才能保证ＥＣ的增殖。

Studies on Embryogenesis and Plant Regeneration of Sweet Potato (Simon.1)(Ipomoea batatas) in Tissue Culture

High frequency somatic embryogenesis and plant regeneration was realized through the leaf culture of "Simon 1", the medium containing MS 2. 4-D 0.5 mg/L KT 0.5 mg/L ABA 5 mg/L was tes- tified to be suitable for the inducing of embryogenic callus. The calluses induced from the leaf had two types: non-embryogenic callus (NEC) and embryogenic callus (EC). NEC grew faster than EC and had no ability to differentiate. In order to enhance the proliferation of the embryogenic callus, NEC and EC must be separat- ed. ABA are essential to induce EC, but higher level of ABA inhibits the constant development of embryoid. When EC was subcultured on medium in which ABA concentration was half of induced medium, the embryoid developed normally. The embryoid developed well on 1/2 MS without growth regulators, plantlets from em- bryoids could grow normally and give high yield.