中药三七根核糖体18S rRNA基因的序列分析

目的：研究中药三七根核糖体18SrRNA基因的序列特征。方法：根据模式植物拟南芥的18SrRNA的基因序列设计引物，对三七根的18SrRNA基因序列进行基因克隆、测序，并与模式植物拟南芥Arabidopsis thaliana以及人参属植物喜马拉雅人参Panax pseudoginseng Wall subsp.himalaicus var.angustifolius的相应序列进行比较。结果：通过对产于广西靖西的三七Panax pseudoginseng Wall.var.notoginseng(Bukill)Hoo et Tseng根的18SrRNA基因进行克隆测序，获得了三七根的18SrRNA基因的部分序列特征。结论：利用已完成全基因组序列测定的模式植物拟南芥的分子生物学信息来研究中药植物基源的基因组序列，将有助于加快中药植物基源的分子生物学研究进程。

Sequencing of ribosomal 18S rRNA gene from Panax pseudoginseng var. notoginseng

Object To identify the characteristics of 18S rRNA from the root of Panax pseudoginsengWall var. notoginseng (Burkill) Hoo et Tseng (PGSN). Methods The primers were designed according to the gene sequence of 18S rRNA from the model plant arabidopsis thaliana. 18S rRNA gene sequence of PGSN were cloned and sequenced and compared with that of the model plant A. thaliana and P. pseudoginseng subsp.Wall himalacus var. angustifolius. Results Part of the characteristics of ribosomal 18S rRNA gene sequence of PGSN from Jingxi, Guangxi Province were identified, which revealed that the 18S rRNA gene sequence of PGSN was 98% similar to that of P. pseudoginseng subsp. himalalaicus var . angustifolius and 96% similar to that of the model plant A. thaliana. Conclusion The use of informations obtained from the model plant, A. thaliana may promote the research progress of molecular biology of TCM drugs.