大肠癌和大肠腺瘤COX-2和BFGF表达的意义

目的:研究COX-2和BFGF在大肠癌及大肠腺瘤组织中的表达及意义．方法:采用免疫组化SP方法检测了手术切除的大肠癌49例,腺瘤性息肉25例,正常大肠黏膜组织20例中的COX-2和BFGF表达．结果:COX-2和BFGF在大肠癌中的表达阳性率分别为59．2％和69．3％,在腺瘤性息肉的表达率为52．0％和56．0％,COX-2和BFGF在大肠癌组织与腺瘤性息肉中表达无显著性差异(P>0．05):正常肠黏膜中未检出COX-2和BFGF．COX-2和BFGF在大肠癌中表达与性别,年龄,肿瘤大小,肿瘤位置,分化程度无关(P>0．05)．但与肿瘤Dukes分期有关,C,D期高于A,B期(81．5％vs 54．5％,P<0．05),淋巴结转移之间具有显著性差,有异淋巴结转移高于无淋巴结转移(81．5％vs 54．5％,P<0．05)．肠癌组织中COX-2、BFGF表达二者间有相关性(r= 0．349,P<0．05)．结论:COX-2、BFGF在大肠癌组织及大肠腺瘤中的表达水平增高,在大肠腺瘤恶变及大肠癌的发生发展过程中起协同作用,共同促进肿瘤的发生．

Expression and significance of cyclooxygenase-2 and basic fibroblast growth factor in colorectal carcinoma and adenoma

AIM: To investigate the mechanism of cyclooxygenase-2 (COX-2), basic fibroblast growth factor (BFGF) in the carcinogenesis of colorectal carcinoma and adenoma. METHODS: Intestinal biopsy specimens were collected from patients with colorectal carcinoma (n = 49), adenoma (n = 25), and normal mucosa (n = 20), and the expression of COX-2 and BFGF were detected by irnmunohistochemi-cal staining (SP method). RESULTS: The positive rates of COX-2, BFGF expression were 59.2%, 69.3% in colorectal carcinoma, and 52.0%, 56.0% in adenoma, respectively. COX-2 and BFGF expression were not detected in normal intestinal mucosa. The positive rates of COX-2 and BFGF expression were not significantly difference between colorectal carcinoma and adenoma (P > 0.05). The expression of COX-2 and BFGF were not significantly correlated with the gender, age, tumor size, tumor location, and cell differentiation, but had marked correlations with Dukes stages (stage C, D vs A, B: 81.5% vs 54.5%, P < 0.05) and lymph node metastasis (metastasis vs non-metastasis: 81.5% vs 54.5%, P < 0.05). Furthermore, there were significant correlations between COX-2 and BFGF expression (r = 0.349, P < 0.05). CONCLUSION: Excessive expression of COX-2 and BFGF may play a synergic role in the carci-nogenesis and development of colorectal carcinoma and adenoma.