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| 胚胎大鼠神经干细胞的培养及鉴定 | |
| 引用本文： | 张清勇,杨波,赵新利,何炜,宋来君,李红伟,冯祖荫,王树凯.胚胎大鼠神经干细胞的培养及鉴定[J].郑州大学学报(医学版),2003,38(1):5-7. |
| 作者姓名： | 张清勇 杨波 赵新利 何炜 宋来君 李红伟 冯祖荫 王树凯 |
| 作者单位： | 1. 郑州大学第二附属医院胸外科,郑州,450003 2. 郑州大学第一附属医院神经外科,郑州,450052 3. 郑州大学基础医学院生理学教研室,郑州,450052 |
| 基金项目： | 河南省医学科技创新人才工程项目 |
| 摘 要： | 目的：探讨胚胎大鼠神经干细胞培养，传及鉴定方法。方法：从胚胎大鼠脑室下区（SVZ）组织分离得到神经干细胞，采用无血清培养技术进行培养，并诱导分化，采用SABC法对分化后后的细胞进行神经元特异性烯醇化酶（NSE）、胶质纤维酸蛋白（GFAP）检测及姬姆萨染色进行细胞鉴定。结果：成功培养出了胚胎大鼠神经干细胞，培养出的细胞具有自我更新能力的增殖能力，可分化为神经元，神经胶质细胞及少突胶质细胞，结论：胚胎大鼠神经干细胞在体外适宜的培养条件下具有自我更新，增殖和多向分化潜能。 |
| 关 键 词： | 神经干细胞 细胞培养 碱性成纤维细胞生长因子 无血清培养技术 |
| 修稿时间： | 2002年11月18 |
Culture and identification of embryonic rat neural stem cells |
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| ZHANG Qingyong,YANG Bo,ZHAO Xinli,HE Wei,SONG Laijun,LI Hongwei,FENG Zuyin,WANG Shukai.Culture and identification of embryonic rat neural stem cells[J].Journal of Zhengzhou University: Med Sci,2003,38(1):5-7. | |
| Authors: | ZHANG Qingyong YANG Bo ZHAO Xinli HE Wei SONG Laijun LI Hongwei FENG Zuyin WANG Shukai |
| Institution: | ZHANG Qingyong,YANG Bo,ZHAO Xinli,HE Wei,SONG Laijun,LI Hongwei,FENG Zuyin,WANG Shukai1 ) Department of Thoracic Surgery,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450003 2 ) Department of Neurosurgery,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 3 ) Department of Physilogy,Zhengzhou University,Zhengzhou 450052 |
| Abstract: | Aim:To incubate,proliferate,and identify the neural stem cells originated from embryonic rat brains. Methods ;The cells isolated from embryonic rat brains were incubated with serum-free medium. The capacity of proliferation and the characteristics of differentiation of the neural stem cells were tested with inlmunocytochemistry. Results: The cells of embryonic rat brains were able to self-renew and proliferate in serum-free medium, and their differentiated offsprings expressed the specific antigens of neurons and glial cells respectively. Conclusion; The neural sphere cells, which were incubated in uitro are able to self-renew,proliferate,and are multipotent. So,they are neural stem cells. |
| Keywords: | neural stem cell embryonic rat cell incubation bFGF |
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