低密度脂蛋白受体基因NcoI多态性与老年人心肌梗死的关系

目的探讨低密度脂蛋白受体基因(LDLR)NcoI多态性与心肌梗死(MI)的相关性,并对其与血脂的关系进行分析。方法用聚合酶链反应(PCR)技术检测102例辽宁藉汉族健康人和84例老年MI患者的LDLR基因NcoI多态性及含量。结果LDLR基因NcoI等位基因频率健康人N+为0.667,MI组基因N+为0.768,MI组比对照组明显增高(P<0.05);血清TC、LDLC、TG及LP(a)水平亦明显增高(P<0.05)。结论NcoI多态性的N+型等位基因与MI的发生密切相关。

Effects of Nco Ⅰ polymorphisms at the LDL receptor gene on myocardial infarction in elderly patients

Objective To investigate the relationship of NcoI polymorphism of LDL receptor gene (LDL-R) with myocardial infarction(MI) in Han elderly patients of Liao Ning province. Methods The polymerase chain reaction (PCR) technique was used to study the polymorphisms of LDL-R gene and allele frequencies in 84 elderly patients with MI and 102 age-matched Chinese healthy controls.The levels of the lipid and lipoproteins were also compared among the cases with MI and the controls. Results N~+ frequencies of LDL-R gene in healthy controls and MI group were 0.667 and 0.768, respectively. The frequency of the genotype N~+N~+(NcoI) was greater in MI group compared with the controls. In the MI group, N~+N~+genotype was associated with high concentrations of total cholesterol and LDL-C, TG, LP(a) in serum. Conclusions The existence of N~+N~+ can affect the concentration of serum lipid level and is closely related with the occurrence of MI.