子宫颈癌有无HPV感染和PCNA，Ki-67，p53表达与临床病理变化相关性分析

目的：探讨子宫颈癌组织HPV感染与PCNA、Ki-67、p53的表达和临床病理资料的相关性。方法76例子宫颈癌组织，来自2012年3月-2012年8月哈尔滨医科大学附属肿瘤医院，所有的病例均有完整的临床资料。采用组织芯片技术、免疫组织化学技术将检测的HPV、PCNA、Ki-67和p53与各项临床病理指标进行比较分析。结果 HPV在子宫颈癌组织中的表达率为68．4％，与淋巴结转移有关（P＜0．01）；PCNA在90．1％子宫颈癌组织中的表达，在35岁以下的妇女子宫颈癌组织中100％表达，与术前是否经过化疗有关（P＜0．01）。 Ki-67表达率为48．9％，p53表达率为38．2％，Ki-67和p53的表达均与淋巴结转移和术前是否化疗有相关性（ P＜0．05或P＜0．01）。结论在子宫颈癌组织中HPV感染、Ki-67和p53的表达与淋巴结转移有相关性。 HPV促进子宫颈上皮细胞的增殖，致抑癌基因p53功能失活，可促进子宫颈癌的侵袭和转移。子宫颈癌术前经过化疗后再行手术，可降低p53、PCNA和Ki-67的表达。

The correlation between HPV infection,the expression of PCNA,Ki -67, p53 and clinicopathological characteristics in cervical carcinoma

Objective To explore the relationship between HPV infection ,the expression of PCNA ,Ki-67,p53 in cervical carcinoma and clinicopathologic data .Methods Seventy-six cases of cervical cancer tissues with complete clinical data were collected from the third affiliated hospital of harbin medical university from march 2012 to September 2012 .Tissue microarray technology ,immunohistochemical technique and statistic method were adopted to make comparative analysis among HPV ,PCNA,Ki-67,and p53 expression in patrents with cervical cancer.Results The expression rate of HPV in cervical carcinoma was 68.4%,which was related to the lym-phatic metastasis（P＆lt;0.05）.Expression of PCNA in cervical cancer tissue was 90.1%and 100%in female pa-tionts women under the age of 35.The expression rate of Ki -67 and p53 were 48.9%and 38.2%respectively, both were correlated to lymphatic metastasis（P＆lt;0.05）and preoperative chemotherapy（P＆lt;0.01）.Conclusion HPV infection with Ki -67,and p53 expression in cervicalcancer tissues are associated with the presence of lymph node metastasis .HPV promotes cervical epithelial cell proliferation ,inactivates tumor suppressor gene p 53 function ,and promotes the invasion and metastasis of cervical cancer .Preoperative chemotherapy of cervical canc-er can reduce the expression of p53,PCNA and Ki-67.