脊神经节微血管内皮细胞阴离子微区的分布特点、生物学特性及损伤的影响

目的探讨脊神经节（ＤＲＧ）血－神经屏障微血管内皮细胞阴离子微区（ＡＭＤ）的分布特点、生物学特性以及与损伤后血管通透性增加的关系。方法健康家兔４２只，分为手术对照组、机械性压迫组、炎性损伤组。采用阳离子胶体金（ＣＣＧ）探针和系列酶消化包埋后标记法观测。结果（１）ＤＲＧ血管内皮细胞上存在着阴离子分布的极化区；（２）涎酸葡萄糖轭合物为ＤＲＧ血管内皮细胞阴离子微区分子的主要成分。结论ＤＲＧ血管内皮细胞对血浆中蛋白仍具有阴离子通透性屏障作用，ＤＲＧ微血管内皮细胞阴离子屏障的损伤将导致微血管通透性的显著增加

Structural and Molecular Characteristics of and Effects of Different Injuries on Anionic Microdomains on Endoneurial Capillaries in Dorsal Root Ganglia

Aim To explore the structural and molecular characteristics of anionic microdomains on the endoneurial capillaries, and the relation with the increased vascular permeability following dorsal root ganglia (DRG) injury. Methods Forty two rabbits were randomly divided into the control group, the mechanical compression group and the inflammatory injury group. Cationic colloidal gold (CCG) probe and various enzymes digestion on CCG labelling were taken for the study. Results (1) There were anionic microdomains and distinct charge polarization in the endotheliocytes of DRG between luminal and albuminal fronts. (2) The sialoglycoconjugates were the major components of the negatively charged microdomains in DGR. Conclusion The endoneurial capillaries in DRG may become a barrier, especially be able to limit the plasma protein extravasation. Inflammatory injury can reduce largely the anionic charges on the luminal plasmalemma, destroy the distribution of anionic microdomains and enhance the vascular permeability significantly.