聚合酶链反应技术快速诊断淋病

作者采用聚合酶链式反应（ＰＣＲ）诊断女性淋病５０例，并将ＰＣＲ与淋菌分离培养比较，结果５０例宫颈分泌物ＰＣＲ检测获得阳性３５例，其中２０例与常规分离培养法一致，另外１５例ＰＣＲ阳性而分离培养为阴性，此１５例临床诊断为淋病，在检测中未见培养阳性而ＰＣＲ检测阴性者，用ＰＣＲ全部实验过程可在５ｈ内完成，较目前分离培养法鉴定淋球菌在时间上明显缩短，所以作者认为ＰＣＲ检测淋菌优于淋菌分离培养，具有简便快速、

^Quick diagnosis of female gonorrhea by polymerase chain reaction

The authors tested the cervical excretions of 50 women by polymerase chain reaction (PCR ) forgonorrhea and compared the results with those tested by gonococcus cultures.The specimens of 35 out of the50 women showed positive reactions to PCR,of which the results of 20 cases were consistent with those bygonococcus cultures,and the other 15 cases were clinically diagnosed gonorrhea, although their culture re-sults were negative,No case of gonococcus culture positive was found PCR negative. PCR result could beavailable in less than 5 h, which was obviously more prompt than that of gonococcus culture. And further-more,it had had the merits of sensitiveness and specificness,Therefore,diagnosing gonorrhea by PCR wasconsidered a better method and worth being recommended for wide application.