用于HCV RNA逆转录聚合酶链反应测定的血清(浆)样本的质量控制

目的 确定用于丙型肝炎病毒（ＨＣＶ）ＲＮＡ测定血清（浆）样本的最佳收集贮存条件。方法 采集ＨＣＶ ＲＮＡ阳性的血样按设定的不同条件处理，用荣光定量ＰＣＲ试剂测定ＨＣＶ ＲＮＡ。结果 血凝集后２小时内离心分离血清ＨＣＶ ＲＮＡ含量无明显改变（降低１０．４２％）；４小时变化明显（降低４０．４９％）。采用抗凝剂的血浆管ＨＣＶ ＲＮＡ含量显著高于血清管（枸橼酸钠抗凝管高出４０．９％，ＥＤＴＡ抗凝管高出５３

Quality contral of plasma(serum) specimen for the detection of HCV RNA with PCR

Objective To define the optimal collection and storage parameters of serum (plasma) forHCV RNA detection. Methods The serum(plasma) obtained from HCV RNA positive patients by differentmeans and different processing and storage conditions, then HCV RNA was detected with the flurescentquantitative PCR. Results The centrifugstion was performed witnin 2 hours aft6r formation of the clot,loss of HCV RNA titers was 10.42% and significant loss of HCV RNA tit6rs 40.49% was observed after4h. The HCV RNA tit6rs in plasma samples with anticoagulants were much higher than that in serumsamples(citrate sodium-anticoagulized 40.97%, EDTA-anticoagLilated 53.14%). There was no loss of HCV RNAwith op to three fi'eeZe-thaw cycles. A significant decrease in HCV RNA(15.6%) was oborved at -20C afterone y6ar. Conclusions The results suggested that it is important to process and store clinical samples forHCV RNA detection. The EDTA-K2 as anticoagUlants the plasma must be collected within 3h, and theserum must be prepared within 2 hours after hemosposia. It is essential to store the sample under -70Cfor the stability of HCV RNA in a longer time.