维生素D对前列腺增生小鼠血清生化指标的影响研究

目的 通过观察维生素D对前列腺增生小鼠动物模型血清生化指标的影响,探讨不同剂量维生素D对前列腺增生动物模型的血清生化指标影响.方法 应用丙酸睾酮肌肉注射制造小鼠前列腺增生模型.随机分组,采用不同剂量的维生素D剂喂养4周后,检测小鼠血清电解质、肝肾功能、血脂等指标.结果 应用0.2、1.0和5.0 μg/kg·d~(-1)的各组小鼠检测结果显示:应用维生素D对前列腺增生小鼠的肾功能无明显负面影响;但是大剂量维生素D对血清谷草转氨酶(AST)有升高作用;中、大剂量维生素D可以升高血清钙离子浓度,对镁、磷无明显影响.结论 中、小剂量维生素D对小鼠前列腺增生模型的肝肾功能、电解质未发现明显负面影响,大剂量维生素D可能对血清钙离子浓度、AST有一定影响.

Influence of vitamin D On the serum biochemical data of benign prostate hyperplasia mice

Objective To investigate the safety of vitamin D on mouse model with benign prostate hyperplasia by analyzing the changes of serum biochemical indexes in benign prostate hyperplasia mice.Methods Mouse model with BPH was established by injecting testosterone.All test mice were randomly divided into different groups.Mice in different groups respectively received different oral doses of vitamin D with 0,0.2,1.0,and 5.0ug/kg·d for four weeks.Levels of serum electrolyte,triglyceride,cholesterol,hepatic function,renal function were detected.Results Levels of serum ALT,AST,BUN,Cr,TG,TC,P,Mg,Ca in mice treated with 0,0.2,1.0 and 5.0ug/kg of vitamin D were as follows:55.2±9.6,54.9±10.1,55.1±11.9,51.3±5.9;188.3±24.7,195.5±31.8,204.9±51.0,232.3±25.9;10.6±1.2,10.4±1.5,8.9±1.1,9.3±1.6;11.3±1.4,11.3±2.9,8.5±5.3,9.8±1.6;1.28±0.38,1.14±0.25.1.08±0.22,1.00±0.31;2.46±0.29,2.45±0.35,2.76±0.68,2.33±035;1.67±0.19,1.69±0.15,1.64±0.17,1.66±0.16;1.66±0.07,1.75±0.19,1.70±0.14,1.68±0.17;and 3.25±O.25,3.33±0.28,3.59±0.22,3.65±0.17.The level of serum AST and the concentration of Ca were upregulated BY high dose of vitamin D(P<0.01),whereas changes of serum Mg and P werenot found Conclusion No injury was found in liver and kidney function of mice with BPH treated with small doses of vitamin D.But the liver function of mice with BPH might be affected by high dose of vitamin D.