番茄红素对心肌细胞氧化应激损伤的保护作用

目的：探讨番茄红素（Lycopene）对心肌细胞氧化应激损伤的保护作用及机制。方法体外培养 H9c2心肌细胞，分为正常对照组（对照组）、番茄红素预处理组（Lycopene组）、模型组（H2 O2组）和番茄红素预处理后建立模型组（Lycopene加H2 O2组）。除对照组及Lycopene组外，其余各组用过氧化氢（H2 O2）200μmol／L处理6 h ，建立心肌细胞氧化应激损伤模型。Lycopene组建模前30 min加入5μmol／L的番茄红素。6 h后M T T观察H9c2心肌细胞存活率；分光光度计检测细胞培养液上清中乳酸脱氢酶（LDH）、肌酸激酶同工酶（CK-MB）活性和细胞中丙二醛（MDA）、超氧化物歧化酶（SOD）活性；激光共聚焦联合流式细胞仪观察细胞内活性氧（ROS）的产生和线粒体膜电位；流式细胞仪检测细胞凋亡；酶标仪检测细胞内ATP水平。结果与对照组比较，H2O2组LDH、CK-BM、ROS、MDA及细胞凋亡率显著增加，差异有统计学意义（P＜0．05）；H9c2心肌细胞存活率、SOD、ATP及线粒体膜电位显著降低，差异有统计学意义（P＜0．05）。与 H2O2组比较，Lycopene加 H2O2组LDH、CK-BM、ROS、MDA及细胞凋亡率显著降低，差异有统计学意义（P＜0．05）；H9c2心肌细胞存活率、SOD、ATP及线粒体膜电位显著增加，差异有统计学意义（P＜0．05）。结论番茄红素可以减轻氧化应激条件下H9c2心肌细胞的损伤，其机制可能与清除细胞内氧化应激产物，增强细胞内抗氧化酶活性及改善线粒体功能有关。

Protective effects of lycopene against oxidative stress-induced injury

Objective To study the protective effects of lycopene pretreatment on H9c2 cardiomyocytes after oxidative stress injured by hydrogen peroxide(H2 O2 ) .Methods H9c2 cardiomyocytes were cultured in vitro and were divided into normal control group ,lycopene treatment group ,model group and lycopene pretreatment+model group .H9c2 cardiomyocytes in model group and Lycopene group were exposed to H2 O2 for 6 h for establishing the model of oxidative stress injury .H9c2 cardiomyocytes in lyco-pene group were pretreated with lycopene for 30 min before subjected to H2 O2 exposure .After the indicated treatment ,the cell via-bility was assessed with MTT kit ,the levels of lactate dehydrogenase(LDH) and creatine kinase-MB(CK-MB) in the culture medi-um ,the levels of superoxide dismutase(SOD) and malondialdehyde(MDA) in H9c2 cells were measured respectively by colorime-try .The reactive oxygen species(ROS) generations and the mitochondrial membrane potential were measured respectively by flow cytometry in combination with laser confocal microscopy .Cardiomyocyte apoptosis was measured by flow cytometry .The ATP con-tents in H9c2 cells and were measured using a microplate reader .Results To compare with control group ,the levels of LDH ,CK-MB ,ROS ,MDA and apoptotic rates in H2 O2 group were increased significantly(P<0 .05) ,while the viability of H9c2 cardiomyo-cytes ,levels of SOD ,ATP and mitochondrial membrane potential were significantly decreased(P<0 .05) .To compare with H2O2 group ,the levels of LDH ,CK-MB ,ROS ,MDA and apoptotic rates in lycopene pretreatment group were decreased significantly(P<0 .05) ,while the viability of H9c2 cardiomyocytes ,levels of SOD ,ATP and mitochondrial membrane potential were significantly in-creased(P<0 .05) .Conclusion Lycopene can provide significant cardioprotective effects against oxidative stress injury .The mecha-nisms might be attributed to scavenging oxidative stress productions ,increasing antioxidant defense enzyme and improving mito-chondrial function .