| 结核分枝杆菌链霉素耐药分离株rpsL基因突变的检测 |
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| 引用本文： | 彭义利,王国斌,张舒林.结核分枝杆菌链霉素耐药分离株rpsL基因突变的检测[J].中华检验医学杂志,2000,23(3):148-149. |
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| 作者姓名： | 彭义利 王国斌 张舒林 |
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| 作者单位： | [1]河南省胸科医院 [2]分子生物学实验室 |
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| 基金项目： | 河南省重大科技攻关项目!资助 (971 2 0 0 1 0 0 ) |
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| 摘 要： | 目的 探讨结核分枝杆菌（结核菌）对链霉素（Ｓｍ）产生耐药性的分子机制，建立直接快速检测结核菌Ｓｍ药物敏感性的试验方法。方法 应用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）银染分析技术检测３２例结核菌临床分离株的ｒｐｓＬ基因。结果 以结核菌标准株（Ｈ３７Ｒｖ）为对照，３２株结核菌临床分离株中，１０株Ｓｍ敏感株的ｒｐｓＬ基因未见ＳＳＣＰ泳动异常，２２株Ｓｍ耐药株中，１６株（７２．７％）的ｒｐｓＬ
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| 关 键 词： | 结核分枝杆菌 链霉素 抗药性 rpsL基因 |
| 修稿时间： | 1999-12-13 |
Detection for the rpsL mutation of streptomycin-resistant M. tuberculosis |
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| PENG Yili,WANG Goubin,ZHANG Shulin,et al..Detection for the rpsL mutation of streptomycin-resistant M. tuberculosis[J].Chinese Journal of Laboratory Medicine,2000,23(3):148-149. |
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| Authors: | PENG Yili WANG Goubin ZHANG Shulin |
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| Institution: | PENG Yili,WANG Goubin,ZHANG Shulin,et al. Laboratory of Molecular Biology,Henan Chest Hospital,Zhengzhou 450003,China |
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| Abstract: | Objectives To study the molecular mechanism of M. tuberculosis resistance to streptomycin, and to set up a method for direct, rapid detection of streptomycin resistance mutations in M. tuberculosis. Method The rpsL genes of 32 M. tuberculosis clinical isolates were tested by PCR SSCP. Results Strain H 37 R v was used as a control. In 32 M. tuberculosis clinical isolates, the rpsL PCR fragments form 10 drug susceptible isolates had no differences in rpsL PCR profiles with strain H 37 R v. In 22 streptomycin resistant isolates,16 showed apparent differences in SSCP profiles.Conclusions The rpsL gene mutation is an important mechanism of M. tuberculosis resistance to streptomycin. PCR SSCP might be used as a simple, rapid and reliable diagnostic method for M. tuberculosis drug resistance. |
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| Keywords: | Mycobacterium tuberculosis Streptomycin Drug resistance Polymerase chain reaction Polymorphism single stranded conformational |
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