HPLC-DAD法同时测定气滞胃痛颗粒中5个成分的含量

目的:建立HPLC法同时测定气滞胃痛颗粒中白芍药苷、芍药苷、甘草苷、柚皮苷和新橙皮苷的含量。方法:采用依利特Hypersil C18色谱柱(4.6 mm×150 mm,5μm),以乙腈(A)-水(B)为流动相梯度洗脱(0～45 min,10%A→28%A),流速1.0mL.min-1,采用DAD检测器在200～400 nm波长处进行检测,柱温30℃。结果:调取不同波长的色谱图分别计算各成分的含量,白芍药苷(230 nm)、芍药苷(230 nm)、甘草苷(237 nm)、柚皮苷(283 nm)和新橙皮苷(283 nm)进样量分别在0.12～6.14μg(r=0.9998),0.40～7.92μg(r=0.9998),0.06～3.18μg(r=1.000),0.20～9.84μg(r=0.9999),0.12～6.14μg(r=0.9999)范围内呈现良好的线性关系。平均回收率(n=6)分别为98.1%,98.3%,98.9%,98.6%,97.1%;RSD分别为0.63%,0.69%,0.96%,1.5%,1.3%。结论:该方法快速简便,结果准确可靠,为气滞胃痛颗粒的全面质量控制提供科学的依据。

HPLC-DAD method for simultaneous determination of five constituents in Qizhi Weitong granules

Objective:To establish an HPLC method for simultaneous determination of five constituents(albiflorin,paeoniflorin,liquiritin,naringin,neohesperidin)in Qizhi Weitong granules.Methods:The separation was performed on an Elite Hypersil C18 column(4.6 mm×150 mm,5 μm)with the gradient elution of acetonitrile(A)-water(B)(0-45 min,10%A→28%A)at a flow rate of 1.0 mL·min-1.The DAD detection wavelength was 200-400 nm.The column temperature was 30 ℃.Results:The contents of albiflorin and paeoniflorin were determined at 230 nm wavelength with good linearity in the range of 0.12-6.14 μg and 0.40-7.92 μg,respectively;the content of liquiritin was determined at 237 nm wavelength with good linearity in the range of 0.06-3.18 μg;The contents of naringin and neohesperidin were determined at 283 nm wavelength with good linearity in the range of 0.20-9.84 μg and 0.12-6.14 μg,respectively.The average recoveries of above five constituents were 98.1%,98.3%,98.9%,98.6% and 97.1%,with RSD of 0.63%,0.69%,0.96%,1.5% and 1.3%,respectively.Conclusion:The developed method is rapid,simple,and accurate,which is helpful for the quality control of Qizhi Weitong granules.