人原核期胚胎的核仁数量与胚胎质量的相关性研究

目的:探讨受精卵核仁的具体数量与受精卵卵裂、可移植胚胎和优质胚胎率间的相关性。方法:精卵结合后16～18h观察受精情况,分别计数雌、雄原核的核仁数目,相对应观察每个受精卵的发育情况。分析两个原核核仁数目之间的比值、和值以及差值与移植当日胚胎质量之间的相关性。结果:雌、雄原核核仁数目之间的比值与移植当日的优质胚胎率存在显著相关。当核仁数的比值>1.25时,受精卵的卵裂率、可移植胚胎和优质胚胎率均有降低趋势。虽然核仁数目之间的差值、和值与卵裂率、可移植胚胎和优质胚胎率均无显著相关,但随着核仁数目之间差值的逐渐增大,受精卵的卵裂率、可移植胚胎和优质胚胎率均有下降趋势;而随着核仁数目之间和值的逐渐增大,受精卵的优质胚胎率有逐渐升高的趋势。结论:受精卵核仁的具体数量与受精卵卵裂、可移植胚胎和优质胚胎率之间存在相关性。核仁的具体数量对预测种植日的胚胎质量有一定的价值。用于移植应选择两个原核间核仁数目比值相对较小的受精卵。

Correlation between the number of pronuclear nucleoli and the developmental competence of human embryos

Objective:To investigate the number of nucleoli in human pronuclear embryos and its correlation with embryonic development competence. Methods:The fertilization information was checked 16-18 h after microinjection or insemination. The numbers of nucleoli of female and male pronuclei were counted and analyzed. Each zygote was cultured individually and embryo morphology was assessed per 24 h till the transfer day. Results:The ratios of female and male pronuclear nucleoli were significantly correlated with the percentages of good quality embryos on the transfer day. Although there were no significant correlation between the difference or sum of nucleoli number of female and male pronuclei and the embryo quality, the percentages of good quality embryos decreased progressively with the number difference of nucleoli of female and male pronuclei, but increased progressively with the number sum of nucleoli of female and male pronuclei. Conclusion:The numbers of nucleoli of female and male pronuclei were correlated with the percentages of good quality embryos, which is valuable in the selection of embryos for transfer.