糖尿病足溃疡大鼠血锌水平及金属硫蛋白的表达意义

目的 探讨糖尿病足溃疡大鼠血锌水平及金属硫蛋白（ metallothionein,MT）的表达对清除自由基及抗氧化作用的意义.方法 将60只雄性Wistar大鼠随机分为对照组（20只）和造模组（40只）,其中造模组链脲佐菌素尾静脉注射诱导糖尿病,对照组注射等量柠檬酸-柠檬酸钠缓冲液.将两组大鼠足背部一矩形全层皮肤组织切除,建立糖尿病足溃疡大鼠模型,监测两组大鼠血锌水平变化,应用免疫组化技术检测MT的表达.结果 造模组大鼠血锌水平（1.17 ±0.09）较对照组（ 1.48±0.07）明显减低,差异有统计学意义（t=12.94,P＜0.01）.免疫组化研究发现在对照组大鼠有少量MT表达,而造模组大鼠MT的表达明显增高,差异有统计学意义（x2=12.78,P＜0.01）.结论 糖尿病足溃疡大鼠的血锌水平及MT的表达情况可能与清除自由基、抗氧化作用有关,MT的表达增高对防止糖尿病足溃疡病变进一步发展可能具有重要作用.

Study on blood zinc levels and significance of metallothionein expression in rats with diabetic foot ulcer

diabetic foot ulcer of rats induced by streptozotosin,and to find the significance of free radical scavenging and the antioxidative function.Methods A total of 60 male wistar rats were randomized into the control group (20 rats) and diabetic model group (40 rats).The model group was induced to diabetic foot ulcer using streptozotosin tail intravenous injection,and the control group was injected equal citric acid-citric acid sodium buffer fluid instead.A rectangular full-thickness skin of dorsum of foot in the two groups was removed from model rats to construct diabetic foot ulcer.Serum zinc levels of the two groups were monitored,and immunohistochemical technique was used to detect the MT expression.Results Serum zinc levels in the model group( 1.17 ±0.09) was significantly lower than control group( 1.48 ±0.07),and the difference was statistically significant ( t =12.94,P ＜0.01 ).The MT expression in control group was low,while it significantly increased in diabetic model group,and the difference was statistically significant ( x2 =12.78,P ＜ 0.01 ).Conclusions The zinc levels and MT expression of diabetic foot ulcer rats may be associated with free radical scavenging and the antioxidative function.The increased expression of MT may play an important role in preventing the further development of diabetic foot ulcer disease.