系统性硬皮病患者外周血CD4^＋T细胞中CD40L表达水平的研究

目的研究系统性硬皮病（SSc）患者外周血CD4^＋T细胞中CIM0配体（CIMOL）的表达水平。方法应用密度梯度离心法分离26例SSc患者（女16例，男10例）和25例正常对照者（女15例，男10例）的外周血单个核细胞，磁珠分选CD4^＋T细胞。RT—PCR检测CD4^＋T细胞中CD40L mRNA的表达水平；流式细胞术检测CD40L蛋白在CD4^＋T细胞中的表达水平。结果SSc女性患者CD4^＋T细胞中CD40L mRNA和CD40L蛋白的表达水平均显著高于正常女性对照组[5．61±1．86vs2．80±0．94，P〈0．01；（6．70±3．55）％vs（2．37±1．39）％，P〈0．05]；SSc男性患者CD40L mRNA和CD40L蛋白的表达水平与正常男性对照组相比差异均无统计学意义[2．59±0．89vs1．92±0．56，P〉0．05；（2．06±1．09）％vs（2．13±0．87）％，P〉0．05]。结论女性SSc患者CD^＋T细胞中CD40L表达显著增高，而男性患者无明显改变，提示CD40L仅在女性SSc发病中起着重要的作用。

Expression of CD40L in CD4 + T cells from patients with systemic sclerosis

Objective To investigate the expression levels of CD40L in CD4 + T cells from systemic sclerosis (SSc) patients.Methods PBMC ( peripheral blood mononuclear cells) cells were isolated from the peripheral venous blood of SSc patients (16females,10males) and healthy donors (15females,10males) by density gradient centrifugation.CD4 + T cells were isolated using magnetic beads.mRNA levels of CD40L in CD4 + T cells were measured by real-time quantitative polymerase chain reaction (RTPCR).Flow cytometric analysis was used to detect the CD40L protein on the surface of CD4 + T cells.Results Both CD40L mRNA and protein expression in CD4 +T cells was significantly elevated in female SSc patients compared with female healthy controls [5.61 ± 1.86 vs 2.80 ±0.94,P ＜0.01 ; (6.70 ±3.55)％vs (2.37 ± 1.39)％,P ＜ 0.05,respectively].No significant increase in CD40L mRNA and protein expression was observed in male SSc patients compared with male controls [2.59 ± 0.89 vs 1.92 ± 0.56,P ＞0.05; (2.06±1.09)％ vs (2.13±0.87)％,P ＞0.05,respectively].Conclusions CD40L was overexpressed in CD4 + T cell of female patients but not males,which maybe one of the important reasons for female susceptibility to SSc.