HMGB1和Smac／DIABLO在上皮性卵巢癌中的表达及意义

目的探讨人类高迁移率族蛋白B1（HMGB1）和Smac／DIABLO蛋白在上皮性卵巢癌中的表达及意义。方法采用免疫组化法和末端脱氧核苷酸转移酶介导缺口末端标记法，检测上皮性卵巢癌（45例）、良性卵巢上皮性肿瘤组织（18例）及正常卵巢组织（20例）中HMGB1和Smac／DIABLO蛋白的表达及细胞凋亡情况，分析其在不同组织中的表达及意义。结果HMGB1蛋白在上皮性卵巢癌中的表达明显高于良性卵巢上皮性肿瘤组织和正常卵巢组织[66．7％（30／45）VS16．7（3／18）、15％（3／17），P〈0．05]；HMGB1蛋白表达与淋巴结转移有关（P〈0．05），与细胞凋亡指数呈负相关（rs=-0．583，P=0．000）；Smac／DIABLO在上皮性卵巢癌中的表达要明显低于良性卵巢上皮性肿瘤组织和正常卵巢组织[20．0％（9／36）vs61．1％（11／18）、80．0％（16／20），P〈0．05]；上皮性卵巢癌组织中HMGB1表达与Smac／DIABLO表达呈负相关（rs=-0．40，P=0．006）。结论HMGB1在上皮性卵巢癌组织中呈过表达，其可能参与了肿瘤的转移；HMGB1与Smac／DIABLO表达呈负相关，HMGB1可能通过影响肿瘤细胞的凋亡失控，参与上皮性卵巢癌的发生发展。

Significance of expression of HMGB1 and Smac/DIABLO protein in epithelial ovarian cancer

Objective The purpose of this study was to investigate the clinical significance of HMGB1 and Smac/DIABLO protein expression in epithelial ovarian cancer.Methods Immunohistochemistry (IHC) and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining were used to detect the protein expression of HMGB1,Smac/DIABLO and cell apoptosis in 45 epithelial ovarian cancers by tissue microarray.Results The protein expression of HMGB1 in epithelial ovarian cancers was significant higher than benign cancers and normal ovarian tissues( P ＜0.05).The protein expression of HMGB1 was related to the lymph node metastasis,which had a negative correlation with the cell apoptosis index ( rs =-0.583,P =0.000).The protein expression of Smac/DIABLO in epithelial ovarian cancers was significantly lower than benign cancers and normal ovarian tissues( P ＜0.05) ; the protein expression of HMGB1 in epithelial ovarian cancers had a negative relationship with protein expression of Smac/DIABLO( rs =-0.40,P =0.006).Conclusions The over-expression of HMGB1 in epithelial ovarian cancers may play an important part in the metastasis of the epithelial ovarian cancers.The over-expression of HMGB1 and the low-expression of Smac/DIABLO may take part in the occurrence and development of the epithelial ovarian cancers through abnormal cell apoptosis.