| 一株产10-羟基喜树碱喜树内生真菌的分离和鉴定 |
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| 引用本文： | 汪学军,闵长莉,刘文博,薄翠英. 一株产10-羟基喜树碱喜树内生真菌的分离和鉴定[J]. 中国抗生素杂志, 2011, 36(6): 407-411 |
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| 作者姓名： | 汪学军 闵长莉 刘文博 薄翠英 |
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| 作者单位： | 皖西学院生物与制药工程系,六安,237012 |
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| 摘 要： | 目的 从喜树组织中筛选产10-羟基喜树碱的内生真菌,并对其进行鉴定.方法 从喜树(Camptotheca acuminate Decne)树皮中分离、纯化得到6株内生真菌,经摇瓶发酵后,采用TLC法、HPLC和MS分析菌丝的提取物;用插片法进行形态学观察,采用分子生物学方法对XK002菌株rDNA的ITS基因(ITS-...
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| 关 键 词： | 喜树内生真菌 分离 鉴定 Valsa mall Miyabe et Yamada 10-羟基喜树碱 |
Isolation and identification of a 10-hydroxycamptothecin-producing endophytic fungus from Camptotheca acuminate Decne |
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| Wang Xue-jun,Min Chang-li,Liu Wen-bo,Bo Cui-ying. Isolation and identification of a 10-hydroxycamptothecin-producing endophytic fungus from Camptotheca acuminate Decne[J]. Chinese Journal of Antibiotics, 2011, 36(6): 407-411 |
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| Authors: | Wang Xue-jun Min Chang-li Liu Wen-bo Bo Cui-ying |
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| Abstract: | Objective To isolate and identify the endophytic fungi which can produce the 10-hydroxy-camptothecin from Camptotheca acuminate Decne.Methods Six endophytic fungi have been isolated from Camptotheca acuminate Decne bark.The organic extracts of mycelium fermented by shake flask were analyzed with thin layer chromatography(TLC),high performance liquid chromatography(HPLC) and electron spray-mass spectra.Cover glass inverted in flat plate was applied to observe morphology character of mycelium.Similarities wer... |
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| Keywords: | Valsa mall Miyabe et Yamada |
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