强噪声暴露后胃溃疡大鼠的血浆多肽激素水平

目的观察强噪声暴露后胃溃疡大鼠血浆多肽激素水平的变化,并讨论其机制.方法制作大鼠胃溃疡模型,进行强噪声刺激,空腹时经眼球静脉取血,采用放射免疫分析法测定血浆激素刎含量,并测量大鼠胃溃疡面积.结果(1)无噪声刺激时,胃溃疡模型术后10 d大鼠血浆胃动素MTL,(160.70±40.34)pg/ml]和骨钙素BGP,(27.63±13.13)pg/ml]水平均明显高于对照组分别为(89.21±49.94)、(9.10±1.38)pg/ml],差异有显著性(P＜0.05),而胃泌素(GAS)水平(107.00±21.75)pg/ml]则明显下降,与对照组(158.48±20.92)pg/ml]的差异亦有显著性(P＜0.01).(2)强噪声刺激时,无溃疡大鼠10 d时的血浆MTL(312.80±207.42)pg/ml]和BGP(17.76±12.33)pg/ml]均明显高于对照组,差异均有显著性(P＜0.01、P＜0.05);而GAS水平无明显变化.(3)胃溃疡大鼠强噪声暴露第10天和第40天的溃疡面积分别为(15.33±7.26)mm2和(15.11±12.45)mm2]较对照组分别为(8.22±6.66)mm2和(3.67±9.90)mm2]增大,差异有显著性(均P＜0.05);而第10天和第40天MTL水平分别为(244.44±68.11)pg/ml和(191.20±60.50)pg/ml]与对照组分别为(160.70±40.34)、(93.10±52.90)pg/ml]比较,均明显升高,且差异均有显著性(均P＜0.01).结论强噪声暴露可使大鼠的溃疡恶化,并对其愈合有明显的负性影响.强噪声暴露和胃溃疡等复合因素作用下,大鼠胃肠内分泌反应明显紊乱.

Plasma polypeptide hormone levels in rats with gastric ulcer after exposure to intense noise

OBJECTIVE: To observe changes of plasma polypeptide hormone levels in rats with gastric ulcer after exposure to intense noise, and to discuss their mechanism. METHODS: 80 Wistar rats were used in the study. Plasma levels of rat gastrin (GAS), motilin (MTL), osteocalcin (BGP), substance P (SP), neurotensin (NT) and somatostatin (SS) in rats were measured by radioimmunoassay. RESULTS: (1) In non-noise-exposure but with gastric ulcer group, the plasma MTL (160.70 +/- 40.34) pg/ml] and BGP (27.63 +/- 13.13) pg/ml] levels on 10 d after gastric ulcer model operation were remarkably higher than those in control group (89.21 +/- 49.94) pg/ml, (9.10 +/- 1.38) pg/ml respectively] (P < 0.05 and P < 0.01), while the GAS level was remarkably descended (107.00 +/- 21.75) vs (158.48 +/- 20.92) pg/ml] (P < 0.01). (2) In noise-exposure but without gastric ulcer group, the plasma MTL (312.80 +/- 207.42) pg/ml] and BGP (17.76 +/- 12.33) pg/ml] levels on 10 d were also significantly increased as compared with the control group (P < 0.01 and P < 0.05 respectively), while the GAS levels didn't change. (3) In noise-exposure + gastric ulcer group, the areas of gastric ulcer on 10 d and 40 d after noise and operation (15.33 +/- 7.26) and (15.11 +/- 12.45) mm(2) respectively] were significantly larger than those of the control (8.22 +/- 6.66), (3.67 +/- 9.90) mm(2)] (P < 0.05). The plasma MTL levels on 10 d and 40 d (244.44 +/- 68.11) and (191.20 +/- 60.50) pg/ml respectively] were higher than those in control group (160.70 +/- 40.34) and (93.10 +/- 52.90) pg/ml respectively] (P < 0.01). CONCLUSION: Intense noise exposure may make the rat gastric ulcer worsened and induce negative effect on healing of it. The gastrointestinal endocrine would be disturbed by combined effect of intense noise exposure with gastric ulcer in rats.