125例地中海贫血杂合子临床血液学分析

目的对１２５例不同类型的地中海贫血（地贫）杂合子进行临床血液学分析。方法用Ｃｏｕｌｔｅｒ血球计数仪测定分析对象末梢血红细胞平均容量（ＭＣＶ）和红细胞平均血红蛋白（ＭＣＨ）。用醋纤薄膜电泳比色法及１分钟碱变性法分别测得ＨｂＡ２和ＨｂＦ含量。结果所有地贫杂合子的ＭＣＶ、ＭＣＨ明显小于正常值（Ｐ＜０．００１），符合小细胞低色素性贫血，同时发现不同的β地贫突变类型，其ＭＣＶ也存在差异，β０突变杂合子ＭＣＶ低于β＋突变杂合子（Ｐ＜０．０３）。结论ＭＣＶ和ＭＣＨ的测定不仅可为地贫的临床诊断提供依据，而且ＭＣＶ还可以作为地贫杂合子类型鉴定的参考

Clinical Hematological Analysis of 125 Heterozygotes for Thalassemias

Objective To analyze the clinical hematological data of 125 heterozygotes for different kinds of thalassemias.Method Mean corpuscular volume (MC V) and Mean corpusular hemoglobin (MCH) of 125 heterozygotes for thalassemias were measured by an automatic Coulter analyzer.Hemoglobin A2 was determined spectrophotometrically after electrophoretic separation on cellulose acetate membrane.Hemoglobin F was determined by alkali denaturation test.Results Mean MCV and MCH in all the tested thalassemia heterozygotes were statistically significantly lower than those in normals (P<0.001).The result also showed that there was a significant difference of the MCV value between different β thalassemia mutations,i.e.,Mean MCV of β0 thalassemia heterozygotes was statistically significantly lower than β+ thalassemia heterozygotes (P<0.03).Conclusions The MCV and MCH are valuable parameters in clinical diagnosis for thalassemia heterozygotes.In addition,MCV provides information for the identification of thalassemia heterozygotes with mutational diversity.