|
|||||
|
|
| 银杏叶提取物对星形胶质细胞增殖周期的影响 | |
| 引用本文： | 赵敬富,张敬军,刘璇玥. 银杏叶提取物对星形胶质细胞增殖周期的影响[J]. 中国临床神经科学, 2011, 19(1): 60-64 |
| 作者姓名： | 赵敬富 张敬军 刘璇玥 |
| 作者单位： | 1. 淄博市桓台县人民医院神经科,256400 2. 泰山医学院附属医院神经科,271000 3. 淄博市职业病防治院,255000 |
| 基金项目： | 山东省中医局课题,泰安市科技局课题 |
| 摘 要： | 目的:探讨银杏叶提取物(EGb)对体外培养的大鼠大脑皮质星形胶质细胞(Ast)增殖周期的影响。方法:体外培养、纯化及鉴定大鼠大脑皮质Ast;MTT比色法选择EGb作用浓度;1×10-7μL·L-1EGb50μL分别加入对照组和实验组培养液;利用流式细胞仪分析技术检测细胞周期。结果:随着Ast培养时间的延长,12h内EGb诱导Ast进入S期+G2/M期的细胞百分率有上升趋势,但24h后稍有下降。结论:EGb可通过影响Ast的细胞周期促进Ast的增殖。 |
| 关 键 词： | 银杏叶提取物 星形胶质细胞 细胞周期 细胞培养 大鼠 |
Effects of Ginkgo Biloba Extract on Cell Increasing Cycle in Astrocytes |
|
| ZHAO Jing-Fu,ZHANG Jing-Jun,LIU Xuan-Yue. Effects of Ginkgo Biloba Extract on Cell Increasing Cycle in Astrocytes[J]. Chinese Journal of Clinical Neurosciences, 2011, 19(1): 60-64 | |
| Authors: | ZHAO Jing-Fu ZHANG Jing-Jun LIU Xuan-Yue |
| Affiliation: | ZHAO Jing-Fu1,ZHANG Jing-Jun2,LIU Xuan-Yue3 1Departmentof Neurology,The People's Hospital of Huantai County,Huantai,Shandong 256400,2Department of Neurology,Taishan Medical College,Taian Shandong 271000,3Zibo Hospital for Occupational Disease Control and Prevention,Zibo Shandong 255000,China |
| Abstract: | Aim：To explore the effects of Ginkgo biloba extract（EGb） on cell increasing cycle in cultured astrocytes from cerebral cortex of rat in vitro. Methods：Astrocytes from rat cerebral cortex were cultured,purified and identified. The concentration of the drugs were selected by MTT assay. 50 μL EGb（1×10-7 μL·L-1） were added to the control group and experimental group respectively. Cell cycle were tested by flow cytometry. Results：During culturing with EGb,the percentage of the astrocytes in cell S＋G2/M phase was increased within 12 hours,but decreased a little after 24 hours. Conclusion：There is direct effect on cell cycle of astrocytes in rats treated with EGb. |
| Keywords: | ginkgo biloba extract astrocyte cell cycle cell culture rat |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |