| FTY720对体外培养MCF-7细胞增殖及凋亡影响的研究 |
| |
| 引用本文： | 张淑芳,刘大程,孙吉凤,台桂香. FTY720对体外培养MCF-7细胞增殖及凋亡影响的研究[J]. 中国实验诊断学, 2011, 15(1): 48-50 |
| |
| 作者姓名： | 张淑芳 刘大程 孙吉凤 台桂香 |
| |
| 作者单位： | 1. 长春医学高等专科学校,生化教研室,吉林,长春,130031
2. 吉林大学白求恩医学院,免疫学教研室,吉林,长春,130021 |
| |
| 基金项目： | 吉林省教育厅"十一五"科学技术研究项目资助课题 |
| |
| 摘 要： | 目的探讨FTY720对体外培养MCF-7细胞增殖及凋亡的影响。方法体外培养MCF-7细胞,将其培养液中加入不同浓度的FTY720,继续培养48小时,MTT法检测MCF-7细胞的增殖情况;瑞士-姬姆萨染色观察细胞形态变化;流式细胞仪检测FTY720对MCF-7细胞的细胞周期、凋亡率的影响。结果当FTY720浓度为6 400 ng/ml时,体外培养MCF-7细胞的增殖受到明显抑制,抑制率为62.0%（P〈0.05）,而且抑制率呈浓度依赖性;镜下可见细胞出现凋亡的形态;流式细胞仪检测分析显示FTY720可将MCF-7细胞阻滞于G1期,并促进其发生凋亡,凋亡率呈浓度依赖性。结论在体外培养的MCF-7细胞培养液中加入一定浓度的FTY720,能明显抑制该细胞的增殖,调节该细胞周期并诱导其凋亡。
|
| 关 键 词： | FTY720 MCF-7细胞 凋亡 抗肿瘤作用 |
Effect of FTY720 on in vitro MCF-7 proliferation and apoptosis reaction |
| |
| ZHANG Shu-fang,LIU Da-cheng,SUN Ji-feng,et al.. Effect of FTY720 on in vitro MCF-7 proliferation and apoptosis reaction[J]. Chinese Journal of Laboratory Diagnosis, 2011, 15(1): 48-50 |
| |
| Authors: | ZHANG Shu-fang LIU Da-cheng SUN Ji-feng et al. |
| |
| Affiliation: | ZHANG Shu-fang1,LIU Da-cheng2,SUN Ji-feng1,et al.(1.Department of Biochemistry,Basic Medical School,Changchun Medicial College,Changchun 130031,China,2.Department of immunology,Bethune College,Jilin University,Changchun 130021,China) |
| |
| Abstract: | Objective To study effect of FTY720 on in vitro MCF-7 proliferation and apoptosis.Methods MCF-7 cells were cultured in vitro and different concentrations of FTY720 were added to its culture medium,continued to culture for 48 hours,determine MCF-7 cell proliferation by MTT assay;observe cell modality by Switzerland-Giemsa staining;determine cell cycle and apoptosis rate by Flow cytometry Detection.Results FTY720 at concentration of 6 400 ng/ml could significantly suppress in vitro MCF-7 proliferation(P<0.05)... |
| |
| Keywords: | FTY720 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
