实验性自身免疫性脑脊髓炎小鼠脑组织和脊髓中脑型肌酸激酶、钙泵和钙中性蛋白酶的变化

目的：观察实验性自身免疫性脑脊髓炎（EAE）小鼠模型脑组织和脊髓中脑型肌酸激酶（CK-BB）、钙泵（CaATPase）和钙中性蛋白酶（calpain）的变化。方法：C57BL/6小鼠随机分为：EAE组（n=9）,用髓鞘少突胶质细胞糖蛋白35-55多肽（MOG35-55）及完全弗氏佐剂混合抗原乳剂免疫小鼠;对照组（n=5）,注射生理盐水。用MOG35-55诱导C57BL/6小鼠,建立（EAE）动物模型（即多发性硬化模型）。观察记录EAE小鼠行为学变化;采用苏木精-伊红染色、LFB髓鞘染色,酶标仪和分光光度计法检测发病高峰期（免疫后第19天）的中枢神经组织病理学变化、CK-BB,CaATPase和calpain酶活性的改变。结果：EAE组与对照组相比：①日均神经功能评分和日累积评分增加（P〈0.01）;②苏木精-伊红染色：中枢炎症细胞浸润明显（P〈0.05）;③LFB染色：髓鞘脱失较多;④CK-BB和CaATPase活性降低,calpain活性增加（P〈0.05）;⑤EAE组小鼠（只）日均累积神经功能评分与CK-BB和CaATPase活性呈负相关（P〈0.05）;⑥EAE组小鼠（只）日均累积神经功能评分与calpain酶活性呈正相关（P〈0.05）。结论：EAE高峰期中枢CK-BB和CaATPase活性降低、calpain活性增高是EAE发生发展的后果,提示EAE时中枢能量代谢和CaATPase、calpain的病理性损害。

Changes of BB Isoenzyme of Creatine Kinase, CaATPase and Calpain in Experimental Autoimmune Encephalomyelitis Mouse Brain and Spinal Cord

Aim:To investigate the changes of BB isoenzyme of creatine kinase(CK-BB),CaATPase and calpain in experimental autoimmune encephalomyelitis(EAE) mouse brain and spinal cord. Methods:C57BL/6 mice were induced into the models of EAE with multiple sclerosis by myelin oligodendrocyte glycoprotein 35-55 (MOG35-55) peptides. Behavioral changes of the EAE mice were observed and recorded. With HE staining,LFB myelin staining,the changes of the central nervous tissues,CK-BB,CaATPase and calpain activity were assayed ...