亚砷酸钠对大鼠外周血淋巴细胞DNA损伤的研究

目的 研究亚砷酸钠(NaAsO2)染毒对大鼠外周血淋巴细胞DNA的损伤作用.方法 Wistar大鼠32只,体质量180～200 g,雌雄各半,按体质量随机分为4组,分别为0(对照)、0.05、0.15、0.45 mg/L NaAsO2染毒组,每组8只.大鼠自由饮用含不同剂量NaAsO2的水,每天测量体质量并记录水消耗量.连续染毒30 d后,眼眶静脉采血,应用单细胞凝胶电泳试验评价大鼠外周血淋巴细胞的损伤程度.结果 0.05、0.15、0.45mg/L染砷组大鼠的体质量增长[(121.00±38.57)、(120.62±42.80)、(125.38±48.68)g]和饮水量[(36.9±6.2)、(37.9±5.8)、(39.3±4.2)ml/d]与对照组[(119.25±47.27)g、(38.4±5.1)ml/d]比较,差异无统计学意义(F值分别为0.040、0.828,P均＞0.05).0.05、0.15、0.45 mg/L染砷组大鼠的外周血淋巴细胞拖尾率[46.25%(185/400)、57.00%(228/400)、64.00%(256/400)]、彗星尾长[(32.89±17.18)、(58.74±36.28)、(77.55±35.73)μm]、尾矩[(6.29±3.74)、(11.20±9.64)、(17.30±12.60)μm]显著高于对照组[39.25%(157/400)、(18.73±15.83)、(2.61±1.05)μm,P均＜0.01];随着染砷剂量的增加,淋巴细胞拖尾率、彗星尾长和尾矩均呈上升趋势.结论 低剂量砷染毒可引起大鼠外周血淋巴细胞DNA的损伤.

Abstract：

Objective To explore the DNA damage in peripheral blood lymphocytes of rats exposed to sodium arsenite. Methods Thirty-two Wistar rats, weighing 180 - 200 g, equal male and female, were randomly divided into 4 groups, 8 in each group. Sodium arsenite 0(control) ,0.05,0.15,0.45 mg/L were given through drinking water for 30 days. Body weight and drinking water consumption were measured every day. Blood were collected and DNA damage in peripheral blood lymphocytes was examined by single cell gel electrophoresis.Results The increase of body mass[( 121.00 ± 38.57), ( 120.62 ± 42.80), ( 125.38 ± 48.68)g]and water intake [(36.9 ± 6.2), (37.9 ± 5.8), (39.3 ± 4.2)ml/d]in 0.05,0.15,0.45 mg/L sodium arsenite groups were compared with the control group[( 119.25 ± 47.27)g, (38.4 ± 5.1 )ml/d], and the difference were not significant (F = 0.040,0.828, all P ＞ 0.05). The tail ratios[46.25%(185/400) ,57.00%(228/400),64.00%(256/400)], tail lengths [(32.89 ± 17.18), (58.74 ± 36.28), (77.55 ± 35.73 ) μm]and tail moments [(6.29 ± 3.74), ( 11.20 ± 9.64),(17.30 ± 12.60)μm]in 0.05,0.15,0.45 mg/L sodium arsenite groups were significantly higher than those of the control group[39.25%(157/400), (18.73 ± 15.83),(2.61 ± 1.05)μm, all P ＜ 0.01], and the tail ratios,tail lengths and tail moments in lymphocytes increased with increased doses of arsenic concentration. Conclusions Low doses of arsenic exposure can induce DNA damage in peripheral blood lymphocytes of rats.

Effect of sodium arsenite exposure on DNA damage of rat peripheral blood lymphocytes

Objective To explore the DNA damage in peripheral blood lymphocytes of rats exposed to sodium arsenite. Methods Thirty-two Wistar rats, weighing 180 - 200 g, equal male and female, were randomly divided into 4 groups, 8 in each group. Sodium arsenite 0(control) ,0.05,0.15,0.45 mg/L were given through drinking water for 30 days. Body weight and drinking water consumption were measured every day. Blood were collected and DNA damage in peripheral blood lymphocytes was examined by single cell gel electrophoresis.Results The increase of body mass[( 121.00 ± 38.57), ( 120.62 ± 42.80), ( 125.38 ± 48.68)g]and water intake [(36.9 ± 6.2), (37.9 ± 5.8), (39.3 ± 4.2)ml/d]in 0.05,0.15,0.45 mg/L sodium arsenite groups were compared with the control group[( 119.25 ± 47.27)g, (38.4 ± 5.1 )ml/d], and the difference were not significant (F = 0.040,0.828, all P ＞ 0.05). The tail ratios[46.25%(185/400) ,57.00%(228/400),64.00%(256/400)], tail lengths [(32.89 ± 17.18), (58.74 ± 36.28), (77.55 ± 35.73 ) μm]and tail moments [(6.29 ± 3.74), ( 11.20 ± 9.64),(17.30 ± 12.60)μm]in 0.05,0.15,0.45 mg/L sodium arsenite groups were significantly higher than those of the control group[39.25%(157/400), (18.73 ± 15.83),(2.61 ± 1.05)μm, all P ＜ 0.01], and the tail ratios,tail lengths and tail moments in lymphocytes increased with increased doses of arsenic concentration. Conclusions Low doses of arsenic exposure can induce DNA damage in peripheral blood lymphocytes of rats.