扩张性心肌病心力衰竭病人外周血干细胞内源性动员的观察

目的观察扩张性心肌病（DCM）心力衰竭患者外周血中CD34-阳性的单个核细胞（CD34-positive mononuclear cells,MNCCD34）的动员及其变化规律。方法观察DCM患者外周血中不同时间段MNCCD34的数量,测量粒细胞集落刺激因子（granulocyte colony-stimulating factor,G-CSF）等5项细胞因子在血浆中不同时间段的变化水平。结果 （1）MNCCD34从心力衰竭后便开始增加,第5-7天达到峰值,然后逐渐呈下降趋势,但在第28天MNCCD34的数目仍然较高。（2）DCM心衰组和对照组MNCCD34数目在第10天时差异有非常显著性（P〈0.001）。（3）外周血中的细胞因子G-CSF显著增高,且于第10天达到峰值。（4）单因素回归分析显示,循环中MNCCD34的数目同血浆中G-CSF的水平密切相关（r=0.379,P〈0.01）。结论 DCM心衰患者外周血中MNCCD34的数目和G-CSF水平是增高的,且二者密切相关。证明DCM心衰后机体存在骨髓动员和自我修复现象。G-CSF是外周血干细胞的动员剂。

Endogenic mobilization of peripheral blood stem cells in patients with acute heart failure resulting from dilated cardiomyopathy

Objective To investigate the mobilization of CD34-positive mononuclear cells(MNCCD34) in the peripheral blood of the patients with acute heart failure resulting from dilated cardiomyopathy(AHF DCM).Methods To examine the quantity of the MNCCD34 after AHF DCM,and the plasma level of granulocyte colonystimulating factor(G-CSF) at different times.Results(1)MNCCD34 increased after the onset of AHF DCM and peaked on day5 to 7,then gradually decreased,but the number on day 28 was still greater than that on day 1....