| 人胎盘贴壁细胞的分离培养及造血相关因子表达 |
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| 引用本文： | 何津,张毅,江小霞,刘刚,刘元林,李荷莲,毛宁.人胎盘贴壁细胞的分离培养及造血相关因子表达[J].中华血液学杂志,2003,24(12):652-654. |
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| 作者姓名： | 何津 张毅 江小霞 刘刚 刘元林 李荷莲 毛宁 |
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| 作者单位： | 1. 吉林大学第一医院妇产科,长春,130021
2. 100850,北京,军事医学科学院基础医学研究所 |
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| 基金项目： | 国家自然科学基金资助项目 ( 3 0 2 712 45 ) |
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| 摘 要： | 目的：探讨人胎盘贴壁细胞(hPDAC)的分离培养及造血相关因子的表达。方法：酶消化法分离培养人胎盘组织中贴壁细胞，并鉴定其生物学特性，用RT-PCR方法检测造血相关因子表达。结果：成功分离培养出hPDAC，并证实其具有间充质干细胞特性，表达多种造血相关因子，包括SCF、FL、G-CSF、GM．CSF、M-CSF和IL-6。结论：hPDAC可为脐血CD34^ 细胞体外扩增提供潜在滋养层。
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| 关 键 词： | 人胎盘贴壁细胞 分离培养 造血相关因子 表达 |
| 修稿时间： | 2002年11月29 |
Isolation and characterization of human placenta derived adherent cells and their hematopoietic growth factor expression |
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| HE Jin,ZHANG Yi,JIANG Xiao-xia,LIU Gang,LIU Yuan-lin,LI He-lian,MAO Ning.Isolation and characterization of human placenta derived adherent cells and their hematopoietic growth factor expression[J].Chinese Journal of Hematology,2003,24(12):652-654. |
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| Authors: | HE Jin ZHANG Yi JIANG Xiao-xia LIU Gang LIU Yuan-lin LI He-lian MAO Ning |
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| Institution: | Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China. |
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| Abstract: | Objective To isolate and culture human placenta derived adherent cells (hPDAC) and assay their hematopoietic growth factor expression. Methods By enzyme digestion, hPDAC were isolated from human placenta tissue and cultured, and their biological characteristics were studied. The hematopoietic growth factor (HGF) mRNA expression of hPDAC was assayed by RT-PCR.Results hPDAC was successfully isolated from human placenta tissue, which was further confirmed as mesenchymal stem cell-like cells. HGF including SCF, FL, G-CSF, GM-CSF, M-CSF and IL-6 were expressed in hPDAC. Conclusion hPDAC could be used as feeder layer for umbilical cord blood CD 34+ cells ex vivo expansion. |
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| Keywords: | Human placenta derived adherent cells Isolation and culture Hematopoietic growth factors |
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