| 大鼠骨髓间充质干细胞体外定向诱导分化为心肌样细胞的实验研究 |
| |
| 引用本文： | 贾绍斌,马萍,刘晓方,仇玉民,徐清斌,戴贵东.大鼠骨髓间充质干细胞体外定向诱导分化为心肌样细胞的实验研究[J].中国心血管杂志,2005,10(5):327-328. |
| |
| 作者姓名： | 贾绍斌 马萍 刘晓方 仇玉民 徐清斌 戴贵东 |
| |
| 作者单位： | 宁夏医学院附属医院心内科,宁夏,银川,750004 |
| |
| 摘 要： | 目的研究大鼠骨髓间充质干细胞的体外培养及向心肌样细胞转化的条件。方法获取成年大鼠胫骨干骨髓,采用贴壁法进行间充质干细胞的培养、传代,观察经5-氮胞苷诱导后骨髓间充质干细胞的生长和分化。结果大鼠骨髓基质细胞贴壁呈集落生长,5-氮胞苷诱导骨髓基质细胞转化为心肌样细胞。结论骨髓基质细胞能够在体外被诱导分化为心肌样细胞,为自体心肌细胞移植提供了一种良好的来源。
|
| 关 键 词： | 骨髓间充质干细胞 细胞培养 |
| 文章编号： | 1007-5410(2005)05-0327-02 |
| 收稿时间： | 2004-12-10 |
| 修稿时间： | 2005-01-24 |
Rat mesenchymal stem cells can be induced into cardiomyocytes in vitro |
| |
| JIA Shao-bin,MA Ping,LIU Xiao-fang,QIU Yu-min,XU Qing-bin,DAI Gui-dong.Rat mesenchymal stem cells can be induced into cardiomyocytes in vitro[J].Chinese Journal of Cardiovascular Medicine,2005,10(5):327-328. |
| |
| Authors: | JIA Shao-bin MA Ping LIU Xiao-fang QIU Yu-min XU Qing-bin DAI Gui-dong |
| |
| Institution: | Department of Cardiology, Affiliated Hospital of Ningxia Medical college, Yinchuan 750004, China |
| |
| Abstract: | Objective To investigate the factors governing the growth and cardiogenic differentiation of rat mesenchymal stem cells in vitro. Methods Mesenchymal stem cells were isolated and culture - expanded, the cardiomyogenic induction was started by adding 5-azaacytidine to the culture medium, and the proliferation and differentiation were observed. Results Mesenchymal stem cells were spindle-shaped and active proliferation capacity in vitro, they earl be differentiation into cardiac-like muscle cells after adding 5-azacytidine. Conclusion Cardiomyocytes can be generated from MSC in vitro. |
| |
| Keywords: | Mesenchymal stem cells Cell culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
