睫状神经营养因子对体外培养的星形胶质细胞细胞周期及Fos蛋白表达的影响

目的研究睫状神经营养因子（CNTF）对体外培养的大鼠大脑皮质星形胶质细胞（Ast）细胞周期及Fos蛋白表达的影响。方法通过体外纯化培养获取大鼠大脑皮质Ast，将CNTF加入培养液，作用相应的时刻点后，应用流式细胞仪测定细胞周期的变化，采用免疫细胞化学方法研究Fos蛋白的表达。结果CNTF作用Ast6垢，可显著促进细胞周期进程，表现为G0／G1期细胞百分比降低；S期＋G2／M期细胞百分比（增殖指数，poliferation index，PI值）升高。12h促增殖作用达高峰，24h、48h有所恢复，但PI仍明显高于对照组。CNTF作用于Ast 2 h后即可引起Fos蛋白的显著表达，并持续至24h，而糖皮质激素预处理可明显抑制Fos蛋白表达。结论CNTF可促进Ast增殖及Fos蛋白表达。

EFFECTS OF CILIARY NEUROTROPHIC FACTOR ON CELL CYCLE AND EXPRESSION OF Fos PROTEIN IN ASTROCYTES IN VITRO

Objective To explore the effects of ciliary neurotrophic factor on cell cycle and expression of Fos protein in cultured astrocytes from cerebral cortex of rat in vitro Methods Astrocytes from cerebral cortex of rat were cultured in vitro.The cultured astrocytes were treated with ciliary neurotrophic factor(CNTF),and then cell cycle was measured by flow cytometer and Fos protein was examined by means of immunocytochemistry at different intervals. Results At 6h following CNTF treatment,astrocytes cell cycle process was promoted obviously,the percentage of cell in G-1/G-0 cycle phase was reduced,while that of G-2/M and proliferation index(PI) increased.The proliferous effect peaked at 12h,and then gradually declined at 24h,48h,but PI was higher than that of control group.The expression of Fos protein was enhanced from 2h to 24h after CNTF treatment,the pretreatment of dexamethasone inhibited the expression of Fos protein.Conclusion CNTF may promote proliferation and the expression of Fos protein in astrocytes.