八氯代二苯并二噁英对小鼠睾丸组织基因表达谱的影响

目的探讨八氯代二苯并二噁英（OCDD）对雄性小鼠睾丸基因表达谱改变的影响。方法20只雄性CBL/6j小鼠，随机分
为对照组（玉米油）、OCDD低剂量组（1.25×10-6 g/mL）、中剂量组（1.25×10-5 g/mL）和高剂量组（1.25×10-4 g/mL），每天灌胃1次，
连续灌胃30 d后睾丸取材，应用基因芯片技术比较不同浓度OCDD染毒组和对照组雄性小鼠睾丸基因表达差异。结果与对照
组相比，低剂量组和对照组差异基因总数1 133个，上调659个，下调474个；中剂量组和对照组差异基因数978个，上调487个，
下调491个；高剂量组和对照组的差异基因数895个，上调424个，下调471个。结论亚慢性OCDD暴露后对小鼠的睾丸相关基
因表达造成影响，提示睾丸可能是OCDD 毒性作用的靶器官。

Effect of 1, 2, 3, 4, 6, 7, 8, 9-octachlorodibenzo-p-dioxin on testicular gene expression profiles in male mice

Objective To investigate the effect of 1,2,3,4,6,7,8,9-octachlorodibenzo-p-dioxin (OCDD) on the testicular gene expression profile in the testis of mice. Methods Twenty male C57BL/6j mice were randomly divided into normal control group (fed with maize oil) and 3 OCDD groups treated with OCDD by gavage for 30 days at low-, moderate-, and high doses of 1.25×10-6, 1.25 ×10-5, and 1.25 ×10-4 g/mL, respectively (8 mL/kg daily). The testicular gene expression profiles of the mice were investigated using gene chip technique and compared between OCDD-exposed groups and the control group. Results Compared with the control group, the mice in low-dose OCDD group showed 1133 differentially expressed genes, including 659 up-regulated and 474 down-regulated ones;in the moderate-dose OCDD group, 978 genes were differentially expressed, including 487 up-regulated and 491 down-regulated ones; in the high-dose group, 895 genes were differentially expressed, including 424 up-regulated and 471 down-regulated ones. Conclusion The effect of sub-chronic exposure to OCDD on testicular gene expression profiles in male C57BL/6j mice indicates that the testis is probably the target organ of OCDD.