| 应用PCR法对临床血标本中人微小病毒B19的检测分析 |
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| 引用本文： | 董庆鸣,魏红山,宋淑静,刘志英,郝鹏,刘顺爱,张剑平,戴旺苏,张四平. 应用PCR法对临床血标本中人微小病毒B19的检测分析[J]. 临床输血与检验, 2005, 7(1): 11-12 |
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| 作者姓名： | 董庆鸣 魏红山 宋淑静 刘志英 郝鹏 刘顺爱 张剑平 戴旺苏 张四平 |
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| 作者单位： | 100011,北京地坛医院;辽宁阜新传染病医院 |
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| 摘 要： | 目的探讨PCR法检测临床血标本中人微小病毒B19(HPV B19)的应用价值。方法根据序列比对结果，在HPV B19核苷酸相对保守区设计引物进行PCR扩增。应用双脱氧链末端终止法对HPV B19阳性PCR产物进行克隆测序。结果应用该法最终检出HPV B19的血清稀释度为10^3。序列比较表明，用本法检出的1株HPV B19阳性标本与标准株(Au株)核苷酸序列同源性为92％。检测肝癌和肝硬化患者血清标本各14例，结果HPV B19阳性分别为8例和5例。结论本法可用于HPV B19的临床诊断和流行病学调查。
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| 关 键 词： | 人微小病毒B19 聚合酶链反应 |
| 文章编号： | 1671-2587(2005)01-0011-02 |
| 修稿时间： | 2004-09-19 |
Polymerase Chain Reaction Assay of Human Parvovirus B19 in Clinical Blood Specimens |
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| DONG Qing-ming,WEI Hong-shan,SONG Shu-jing,et al.. Polymerase Chain Reaction Assay of Human Parvovirus B19 in Clinical Blood Specimens[J]. Journal of Clinical Transfusion and Laboratory Medicine, 2005, 7(1): 11-12 |
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| Authors: | DONG Qing-ming WEI Hong-shan SONG Shu-jing et al. |
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| Affiliation: | DONG Qing-ming,WEI Hong-shan,SONG Shu-jing,et al. Beijing Ditan Hospital,Beijing 100011 |
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| Abstract: | Objective To detect human parvovirus B19 in clinical blood samples by polymerase chain reaction (PCR). Methods The primers was derived from the conserved region of HPV B19 according to the result of sequence comparing. The product of the PCR was cloned into plasmid and sequenced by the dideoxy-mediated chain-termination method. Results The maximum dilution of serum was 10 3 for detection of HPV B19 by PCR. Sequence analysis showed nucleotide homology was 92% between the cloned HPV B19 and the standard strain (Au strain) registered in GenBank. The rate of HPV B19 infections were 8/14 and 5/14 in patients with hepatocellular carcinoma and hepatocirrhosis, respectively. Conclusion This method may be used for the clinical diagnosis and epidemiological study of HPV B19 infection. |
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| Keywords: | Human parvovirus B19 Polymerase chain reaction |
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