Ultrastructural Analysis of Apoptosis Induced by the Monoclonal Antibody Anti-APO-1 on a Lymphoblastoid B Cell Line |
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| Authors: | Heinz-RÜ diger K hler Jens Dhein Gerd Alberti Peter H. Krammer |
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| Affiliation: | a Department of Morphology and Ecology, Zoological Institute I, University of Heidelberg, Heidelberg, Federal Republic of Germanyb Institute for Immunology and Genetics German Cancer Research Center, Heidelberg, Federal Republic of Germany |
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| Abstract: | Apoptosis (programmed cell death) is the most common form of death in eukaryotic cells. We recently described a monoclonal antibody, anti-APO-1, which induces apoptosis of cells from the human B-lymphoblastoid line SKW 6.4 and of cells from a variety of other human lymphoid cell lines. This model of apoptosis was now studied ultrastructurally.
SKW 6.4 cells undergoing apoptosis showed the following morphological changes: condensation of the cytoplasm and karyoplasm, formation of large electron-opaque aggregates of the chromatin lining the nuclear membrane, “blebbing” of the cell membrane at an early stage of apoptosis, and dilatation of the mitochondria. Two hours after adding anti-APO-1, the nuclear membrane was ruptured. Occasionally, large vesicular enlargement of endoplasmic reticulum or Golgi appeared in the cytoplasm. Finally, total breakdown of all cell membranes and cellular disintegration was observed. |
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| Keywords: | apoptosis B-lymphoblastoid cells monoclonal antibody anti-APO-1 ultrastructure |
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