应力缺失性骨质疏松模型大鼠p38信号通路以及通络生骨胶囊的干预

背景:应力缺失是导致运动不足人员骨质疏松发生的主要原因。通络生骨胶囊具有促进骨生成的作用,其对应力缺失模型大鼠p38通路的影响未见报道。目的:观察应力缺失性骨质疏松大鼠p38的变化,以及通络生骨胶囊对其改善作用。方法:将SD大鼠随机分为4组,除正常组外以鼠尾悬吊法制备动物应力缺失模型,药物低、高剂量组从造模次日起每日灌胃给予0.6,1.2g/kg的通络生骨胶囊。造模21d,取腿骨,进行形态学观察;分离成骨细胞,Western blot法测定p38蛋白含量。结果与结论:与正常组比较,模型组成骨细胞显著减少,骨小梁面积及其百分比、骨小梁厚度等显著降低,p38表达总量显著升高,但磷酸化p38与对照组无差异。高剂量的通络生骨胶囊可显著增加应力缺失大鼠成骨细胞个数,增大其骨小梁面积,促进磷酸化p38的表达;而低剂量的通络生骨胶囊则降低了磷酸化p38的表达。说明应力缺失可导致大鼠骨质疏松,高剂量的通络生骨胶囊可通过增强p38磷酸化水平预防应力缺失导致的骨质疏松。

Interventional effect of Tongluo Shenggu capsules on expression of p38 signal pathway in stress absence induced osteoporosis rats

BACKGROUND:Stress absence is the main reason for osteoporosis in insufficient exercises people.Tongluo Shenggu capsules can promote bone formation,however,its effect on p38 signal pathway remains poorly understood.OBJECTIVE:To investigate the influence of stress absence on p38 expression and the intervention of the Tongluo Shenggu capsules.METHODS:A total of 40 SD rats were randomly divided into four groups.Exception those in the normal control group,all animals were subjected to tail suspension test for preparing stress absence models.Animals in the drug groups were administrated Tongluo Shenggu capsules(0.6 or 1.2 g/kg) once per day.After model preparation,animals were sacrificed and bones of lower leg were observed.The p38 protein level was determined by Western blot.RESULTS AND CONCLUSION:Compared with the normal control group,the area and thickness of bone tranecula,the amount of osteoblast were decreased obviously in the model group;stress absence induced an increase of the total p38,but had little influence to the amount of phosphorylation p38.High-dose Tongluo Shenggu capsules increased the phosphorylation p38,while low-dose decreased the amount of phosphorylation p38.Stress absence induces osteoporosis,but high-dose Tongluo Shenggu capsules can prevent from osteoporosis via enhancing expression of p38.