同种异体骨髓间充质干细胞移植治疗急性肝功能衰竭

背景:对于急性肝功能衰竭的临床治疗,目前尚缺乏特效手段。骨髓间充质干细胞在特定环境下可分化为具有部分肝功能的类肝样细胞,从而参与肝功能的修复和重构,为急性肝功能衰竭的治疗提供了新的手段。目的:评价同种异体骨髓间充质干细胞移植治疗大鼠急性肝功能衰竭的疗效,为其临床应用提供理论依据。方法:采用全骨髓培养法培养并纯化其骨髓间充质干细胞。30只健康SD大鼠随机均分为3组:正常对照组:不予任何处理;肝衰竭组和移植组:用腹腔注射四氯化碳石蜡油溶液的方法复制鼠急性肝功能衰竭模型后24h分别经其尾静脉注射生理盐水和等量骨髓间充质干细胞。在移植后第1,2,3,7天抽血检测其肝功能,并取肝脏行病理学检查。结果与结论:急性肝功能衰竭鼠经骨髓间充质干细胞移植治疗后生存率为70%,与肝衰竭组大鼠存活率20%相比差异有显著性意义(P<0.05);肝功能指标中谷丙转氨酶和谷草转氨酶相比,移植组明显低于肝衰竭组,差异有显著性意义(P<0.05)。肝脏组织病理学结果显示移植组肝细胞变性及坏死程度以及炎症浸润程度轻于肝衰竭组。因此,经尾静脉移植骨髓间充质干细胞能提高急性肝功能衰竭大鼠的生存率、改善肝功能及减轻肝脏坏死程度,对大鼠急性肝功能衰竭有一定的治疗作用。

Allogeneic bone marrow mesenchymal stem cells transplantation in treatment of acute liver failure

BACKGROUND:Effective therapies are deficient in treating acute liver failure(ALF).At present,there is no effective method.Bone mesenchymal stem cells(BMSCs) can differentiate into hepatocyte-like cells and participate in liver regeneration and repair,which provides a new manner for treatment of ALF.OBJECTIVE:To evaluate the effect of BMSCs transplantation on the ALF in rats,to provide empirical study foundation for future clinical application.METHODS:The BMSCs were cultured and purified by the whole marrow-cultured method.A total of 30 healthy Sprague-Dawley rats were randomly assigned to three groups.Normal control group:no treatment.ALF group and transplantation group:ALF models of rats were developed by intraperitoneal injection with CCL4.24 hours following model induction,physiological saline and an equal volume of BMSCs were injected via the caudal vein.Finally,liver function was tested at 1,2,3,7 days intervals and histopathologic examinations were performed.RESULTS AND CONCLUSION:Following BMSCs transplantation,the survival rates of the rats were increased to 70%,compared to the ALF group(20%),and the difference was statistically significant(P < 0.05).The activities of alanine aminotransferase and aspartate aminotransferase were significantly lower in the transplantation group compared with ALF group(P < 0.05).In pathohistology,the degeneration and necrosis of hepatic cells and degree of inflammation were less obvious than that of ALF group.Hence,BMSCs transplantation via caudal vein could elevate the survival rate of ALF rats,improve hepatic function and alleviate the degree of cell necrosis,and could help to treat ALF in rats.