富血小板血浆临床应用及其生物材料性能

背景:富血小板血浆的制备方法、设备及应用手段等方面有了长足发展,但是相对于国内临床工作者而言富血小板血浆技术仍是一项相对较新的生物技术。目的:对富血小板血浆分类等相关概念和存在问题加以说明和论述,使其制备和应用技术等更加清晰和明确。方法:以"platelet rich plasma,preparation,富血小板血浆,制备"为检索词,检索PubMed数据库及维普数据库1995-09/2010-09相关文章。结果与结论:根据所含白细胞的多少可以将富血小板血浆分为贫白细胞的富血小板血浆和富白细胞的富血小板血浆;根据应用形式又可以分为未激活富血小板血浆和激活的富血小板血浆,后者又可以分为富血小板血浆凝胶和富血小板血浆释放物。被视为第二代血小板浓缩物的富血小板纤维蛋白与富血小板血浆都含有高浓度的血小板,但两者在制备方法,凝胶形成方式等方面有根本的区别。由于其安全性和易于制备,富血小板血浆在医学领域的应用将会越来越多,但临床应用还要保持谨慎,因为富血小板血浆在多个方面还缺乏相关研究。

Clinical application and biomaterial properties of platelet-rich plasma

BACKGROUND:The preparation methods,equipment and application means of platelet-rich plasma(PRP) have been considerably developed,but PRP is still a relatively new biotechnology for the domestic workers.OBJECTIVE:To illustrate and discuss the concepts and existed problems of PRP,to clearly explicit the preparation and application technology of PRP.METHODS:A search across the databases of Pubmed and VIP(from September 1995 to September 2010) was performed,with key words of "platelet rich plasma,preparation" in English and in Chinese.RESULTS AND CONCLUSION:According to the number of white blood cells contained in PRP,PRP can be divided into pure PRP and leukocyte-rich PRP;PRP can also be divided into non-activated PRP and activated PRP depending on the application form.Activated PRP mainly includes PRP gel and releasates.Platelet-rich fibrin,which is regarded as second-generation platelet concentrate,and PRP both contain high concentrations of platelets,but they are fundamentally different in the preparation method and gel formation method.Because of its safety and easy preparation,PRP will be more and more applied in clinical medicine.However,clinical application should be cautious,because many aspects of PRP are still lack of related research.