天然、重组水蛭素对随意皮瓣淤血模型血管内皮细胞生长因子的影响

背景:随意皮瓣移植后易发生皮瓣静脉淤血,甚至坏死,临床与动物实验证明水蛭素能够提高淤血皮瓣的成活率。目的:观察水蛭素对大鼠随意皮瓣淤血模型成活保护作用。方法:Wistar大鼠30只背部设计随意皮瓣10cm×3cm制成淤血模型,随机分为天然水蛭素组、重组水蛭素组和对照组,分别于皮下注射5U天然水蛭素、5U重组水蛭素和生理盐水。术后测定血管内皮细胞生长因子含量及血管内皮细胞生长因子mRNA表达量,计算皮瓣成活率。结果与结论:天然水蛭素组与重组水蛭素组皮瓣成活率比对照组高(P<0.05)。天然水蛭素组与重组水蛭素组比对照组新生毛细血管血管内皮细胞生长因子表达多(P<0.05),且天然水蛭素组最多。提示天然、重组水蛭素均能够促进随意皮瓣血管内皮细胞生长因子表达,有利于新生血管的生成,能够提高随意皮瓣的成活率,且天然水蛭素效果优于重组水蛭素。

Effect of natural and recombinant hirudin on vascular endothelial cell growth factor in random skin flap congestion models

BACKGROUND:Flap venous congestion,even necrosis,occurs after random skin flap transplantation.Clinical and animal experiments have proved that hirudin can improve the survival rate of congestion flap.OBJECTIVE:To investigate the protective effect of hirudin on random skin flap congestion.METHODS:Thirty Wistar rats were established animal model of venous congestion.Dorsal random skin flap was prepared on each animal with 10 cm × 3 cm in size.The models were randomly divided into the natural hirudin,recombinant hirudin and control groups,which received subcutaneous injection of 5 U natural hirudin,recombinant hirudin or physiological saline,respectively.Vascular endothelial cell growth factor(VEGF) levels and VEGF mRNA expression were measured after surgery.The flap survival rate was calculated.RESULTS AND CONCLUSION:Compared with the control group,the flap survival rate of the natural hirudin or recombinant hirudin group was higher(P < 0.05).The VEGF expression of the natural hirudin or recombinant hirudin group was greater than that of the control group(P < 0.05),especially greatest in the natural hirudin group.The results demonstrated that,both natural and recombinant hirudin can promote VEGF expression in random flaps,accelerate angiogenesis and increase survival rate of random flaps.The natural hirudin shows superior effects to recombinant hirudin.