放射性咀嚼肌损伤模型软组织病理改变及转化生长因子β1的表达

背景:长期的放射线累积更可引起周围组织纤维化,张口受限,严重影响肿瘤患者的生活品质.目的:观察大鼠咀嚼肌放射损伤后软组织的病理改变及体内转化生长因子β1 mRNA的表达.方法:成年Wistar大鼠30只,随机分成对照组(n=10)和放射组(n=20).放射组利用直线加速器累积照射咀嚼肌40 Gy.光镜和电镜下观察咀嚼肌放射损伤区血管病理改变及RT-PCR检测咀嚼肌放射损伤区转化生长因子β1基因表达.结果与结论:放射组咀嚼肌放射损伤区毛细血管密度较对照组明显降低(P < 0.01),转化生长因子β1 mRNA水平较对照组显著升高(P < 0.01).提示放射损伤可促使组织纤维化修复放射损伤.

Pathological changes of soft tissues and transforming growth factor beta 1 expression in radiation masseter injury models

BACKGROUND: Long-term radioactive ray accumulation leads to surrounding tissues fibrosis accompanied by limitation of mouth-opening, which seriously affects life quality of tumor patients. OBJECTIVE: To observe pathological change of soft tissues in rats with radiation masseter injury and the level of transforming growth factor β1 (TGF-β1) mRNA expression in vivo.METHODS: A total of 30 Wistar rats were randomly divided into control group (n=10) and radiated group (n=20). Radiated group were radiated with linear accelerator at a dose of 40 Gy. The pathological changes of vessels were observed under light microscope and electron microscope and expression of TGF-β1 was detected by RT-PCR. RESULTS AND CONCLUSION: The microvessel density of radiated group was obviously decreased than that of the control group (P < 0.01), but the mRNA expression of was higher than that in the control group (P < 0.01). It suggests that radiated injury can induce fibrosis to repair the radiated injury.