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Molecular method for Bartonella species identification in clinical and environmental samples |
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| Authors: | García-Esteban Coral Gil Horacio Rodríguez-Vargas Manuela Gerrikagoitia Xeider Barandika Jesse Escudero Raquel Jado Isabel García-Amil Cristina Barral Marta García-Pérez Ana L Bhide Mangesh Anda Pedro |
| Affiliation: | Coral García-Esteban, Horacio Gil, Manuela Rodríguez-Vargas, Xeider Gerrikagoitia, Jesse Barandika, Raquel Escudero, Isabel Jado, Cristina García-Amil, Marta Barral, Ana L. García-Pérez, Mangesh Bhide, and Pedro Anda |
| Abstract: | A new, efficient molecular method for detection of Bartonella, based on the 16S-23S rRNA intergenic spacer and 16S rRNA amplification by multiplex PCR combined with reverse line blotting, was designed. This assay could simultaneously detect 20 different known species and other Bartonella species not described previously. |
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