| TRAIL在重型肝炎患者PBMC和血清中的表达及血浆置换治疗前后的变化 |
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| 引用本文： | 魏屏,张景辉,刘薇,朱祥珍. TRAIL在重型肝炎患者PBMC和血清中的表达及血浆置换治疗前后的变化[J]. 世界华人消化杂志, 2011, 0(19) |
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| 作者姓名： | 魏屏 张景辉 刘薇 朱祥珍 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院感染科;华中科技大学同济医学院附属协和医院外科实验室;华中科技大学同济医学院附属协和医院感染科实验室; |
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| 摘 要： | 目的:探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)在重型肝炎发病机制中的作用以及血浆置换治疗前后变化的意义.方法:采用实时荧光定量PCR反应检测30例重型肝炎患者外周血单个核细胞(PBMC)TRAILmRNA的水平,采用ELISA法检测其血清可溶性TRAIL(sTRAIL)的水平,并与肝功能相关指标进行相关性分析.观察血浆置换(PE)治疗前后TRAIL的变化,比较治疗有效组和治疗无效组之间TRAIL水平差异.结果:重型肝炎患者PBMCTRAILmRNA的水平及血清sTRAIL的水平均明显高于健康对照组(0.0622±0.0227vs0.0059±0.0023,P<0.05;9.1058±3.2260vs2.4552±1.7485,P<0.01).mTRAIL和sTRAIL与肝功能相关指标总胆红素(TB)、白蛋白(ALB)以及凝血酶原时间(PT)均无直线相关性,膜型TRAIL(mTRAIL)和sTRAIL之间也无直线相关性.PE治疗后,肝功能相关指标改善;PBMCTRAILmRNA水平下降,治疗前后有显著性差异(0.0622±0.0227vs0.0214±0.0140,P<0.001);而血清sTRAIL水平...
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| 关 键 词： | 肿瘤坏死因子相关凋亡诱导配体 外周血单个核细胞 重型肝炎 血浆置换 |
Changes in levels of TRAIL mRNA in PBMCs and sTRAIL in serum in patients with severe hepatitis between before and after plasma exchange therapy |
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| Ping Wei,Xiang-Zhen Zhu. Changes in levels of TRAIL mRNA in PBMCs and sTRAIL in serum in patients with severe hepatitis between before and after plasma exchange therapy[J]. World Chinese Journal of Digestology, 2011, 0(19) |
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| Authors: | Ping Wei Xiang-Zhen Zhu |
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| Affiliation: | Ping Wei,Xiang-Zhen Zhu,Department of Infectious Diseases,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hu-bei Province,China Jing-Hui Zhang,Surgical Laboratory,Hubei Province,China Wei Liu,Laboratory for Infectious Diseases,China |
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| Abstract: | AIM:To investigate the role of TNF-related apoptosis-inducing ligand(TRAIL)in the pathogenesis of severe hepatitis and to detect the impact of plasma exchange therapy on the levels of TRAIL. METHODS:The expression of membrane-bound TRAIL in peripheral blood mononuclear cells(PBMCs)was examined by real-time fluorescence quantitative PCR,while serum levels of soluble TRAIL(sTRAIL)were measured by ELI-SA.Correlation analysis was performed among serum TBIL,ALB and PT.Furthermore,the levels of TRAIL in patients ... |
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| Keywords: | Tumor necrosis factor-related apoptosis-inducing ligand Peripheral blood mononuclear cells Severe hepatitis Plasma exchange |
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