| P21对胃癌细胞MGC-803的增殖抑制及对POLD1基因表达的调控 |
| |
| 引用本文： | 阮细玲,李永继,吴琼,廖柳凤,徐恒.P21对胃癌细胞MGC-803的增殖抑制及对POLD1基因表达的调控[J].世界华人消化杂志,2011(19). |
| |
| 作者姓名： | 阮细玲 李永继 吴琼 廖柳凤 徐恒 |
| |
| 作者单位： | 广西医科大学;广西医科大学医学实验中心; |
| |
| 基金项目： | 国家自然科学基金资助项目,No.30950028; 广西自然科学基金资助项目,No.2010jjC40020; 广西医科大学实验中心开放课题基金资助项目,No.KFJJ2010-66~~ |
| |
| 摘 要： | 目的:研究P21对POLD1基因的调控通路,以探索阻断癌细胞恶性增殖的机制.方法:实验主要分3组:阴性对照组(转染空载体pXJ41-neo的803-pXJ细胞)、空白对照组(胃癌细胞MGC-803)、实验组(转染P21重组真核表达质粒pXJ41-p21的803-p21细胞).MTT实验分析细胞增殖变化,流式细胞仪检测细胞凋亡水平,实时荧光定量PCR技术检测基因表达水平的变化,Westernblot分析蛋白表达差异.结果:MTT实验显示,与空白对照和阴性对照组相比,实验组细胞增殖受到明显抑制,凋亡率(%)增高(11.36±0.51vs7.39±0.17,7.69±0.47,F=85.338,均P<0.05).实验组P21mRNA表达水平显著提高(2.15±0.23vs1.05±0.11,1.00±0.00,F=59.054,均P<0.05),POLD1则显著下调(0.45±0.07vs1.09±0.13,1.00±0.00,F=49.907,均P<0.05);P21、P125蛋白表达变化与基因变化相一致.cyclinE、Rb1基因表达均上调,CDK2基因表达下调,c-myc基因表达则变化不大.结论:P21抑制了...
|
| 关 键 词： | 胃癌 P21 POLD1 表达调控 |
P21 suppresses cell proliferation and downregulates POLD1 expression in human gastric cancer cell line MGC-803 |
| |
| Xi-Ling Ruan,Yong-Ji Li,Qiong Wu.P21 suppresses cell proliferation and downregulates POLD1 expression in human gastric cancer cell line MGC-803[J].World Chinese Journal of Digestology,2011(19). |
| |
| Authors: | Xi-Ling Ruan Yong-Ji Li Qiong Wu |
| |
| Institution: | Xi-Ling Ruan,Yong-Ji Li,Qiong Wu,Department of Pathology,Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,ChinaLiu-Feng Liao,Department of Pharmacology,China Heng Xu,Medical Experimental Research Center,China |
| |
| Abstract: | AIM:To investigate whether and how P21 regulates POLD1 expression in human gastric cancer cell line MGC-803 and to find new clues to blocking the malignant proliferation of cancer cells. METHODS:MGC-803 cells were divided into three groups:blank control group(untransfected cells),negative control group(cells transfected with an empty vector pXJ41-neo),and experimental group(cells transfected with a eukaryotic expression plasmid pXJ41-p21).After transfection,cell proliferation was detected by MTT assay;cell ... |
| |
| Keywords: | Gastric cancer P21 POLD1 Expression regulation |
| 本文献已被 CNKI 等数据库收录！ |
|
