| 软脂酸对酒精体外诱导的脂肪变性肝细胞的作用及机制 |
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| 引用本文： | 王春晖,卿笃信.软脂酸对酒精体外诱导的脂肪变性肝细胞的作用及机制[J].世界华人消化杂志,2011(19). |
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| 作者姓名： | 王春晖 卿笃信 |
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| 作者单位： | 中南大学湘雅二医院消化内科; |
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| 摘 要： | 目的:探讨不同浓度的软脂酸对酒精体外诱导的脂肪变性肝细胞的作用及机制.方法:体外培养人正常肝细胞株L-02,设立空白对照组、酒精诱导组及软脂酸干预组.软脂酸干预组设6个浓度梯度(2.5、5、10、20、30、40μmol/L),空白对照组用正常培基培养96h,酒精诱导组和软脂酸干预组在细胞培养24h后加入终浓度为60mL/L的无水乙醇,继续培养24h后,软脂酸干预组加入各浓度软脂酸.采用MTT法测细胞增殖,油红O染色观察细胞内脂滴形成情况,试剂盒检测细胞内三酰甘油的含量,Westernblot法测定细胞核内成熟的固醇调节元件结合蛋白-1c(nSREBP-1c)的含量.结果:60mL/L的无水乙醇培养L-02细胞72h成功建立了脂肪变性模型,软脂酸干预后,软脂酸浓度≤10μmol/L时,软脂酸明显地促进酒精诱导的脂肪变性肝细胞的增殖(P<0.05),并且明显地减轻细胞内脂滴的形成,减少细胞内三酰甘油的含量和细胞核内nSREBP-1c的含量,并呈现出剂量效应关系;而软脂酸浓度≥20μmol/L时,明显地抑制酒精诱导的脂肪变性肝细胞的增殖(P<0.05),并且加重细胞内脂滴的形成,增加细胞内三酰甘油和细胞核内nSR...
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| 关 键 词： | 乙醇 L-02细胞 酒精性脂肪肝 软脂酸 细胞核内成熟的固醇调节元件结合蛋白-1c |
Effect of palmitic acid on ethanol-induced fatty degeneration in hepatic cells in vitro |
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| Chun-Hui Wang,Du-Xin Qing.Effect of palmitic acid on ethanol-induced fatty degeneration in hepatic cells in vitro[J].World Chinese Journal of Digestology,2011(19). |
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| Authors: | Chun-Hui Wang Du-Xin Qing |
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| Institution: | Chun-Hui Wang,Du-Xin Qing,Department of Gastroenterology,the Second Xiangya Hospital of Central South University,Changsha 410011,Hunan Province,China |
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| Abstract: | AIM:To determine the effect of different concentrations of palmitic acid on alcohol-induced lipogenesis in L-02 liver cells and to explore mechanisms involved. METHODS:Cultured L-02 liver cells were divided into three groups:blank control group,alcohol induction group,and palmitic acid intervention group,which were cultured in RPMI 1640 medium containing 10%serum,RPMI 1640 medium containing 10%serum and 6%ethanol,and RPMI 1640 medium containing 10%serum,6%ethanol and different concentrations of palmitic aci... |
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| Keywords: | Ethanol L-02 hepatocytes Alcoholic fatty liver Palmitic acid Nuclear sterol regulatory element-binding protein-1c |
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