穴位埋线对溃疡性结肠炎大鼠ANCA作用的实验研究

目的:观察穴位埋线对溃疡性结肠炎的疗效,并探讨其作用机理.方法:用2,4二硝基氯苯法[1]造模,造模结束后,第20天将造模组随机分为模型组、柳氮磺胺吡啶(SASP)治疗组和穴位埋线治疗组.SASP组用4.5%柳氮磺胺吡啶悬液胃饲治疗,穴埋组采用"足三里""上巨虚"穴内埋线治疗.用间接免疫荧光法检测抗中性粒细胞胞浆抗体(ANCA),病理组织切片技术观察病变.结果:溃疡性结肠炎的实验大鼠ANCA阳性率达62.5%,各组间差异有显著意义(P＜0.05),通过治疗后,两组ANCA阳性率均降低(P＜0.05,P＜0.01).结论:穴位埋线治疗溃疡性结肠炎疗效确切,其作用机理可能与降低NACA有关.

Experimental study on action of acupoint embedding thread on ANCA in the rat of ulcerative colitis

Objective To observe therapeutic effect of acupoint embedding thread therapy on ulcerative colitis and to study the mechanism. Methods The rat model of ulcerative colitis was developed with 2,4-dinitrochlorobenzene method. The 20th day after the end of model development, they were randomly divided into a model group, a salicylazosulfapyridine (SASP) treatment group and an acupoint embedding thread therapy group. The SASP treatment group were treated with intragastrical perfusion of 4. 5% SASP suspension, and the acupoint embedding thread therapy group were treated with embedding thread at "Zusanli" (ST 36) and "Shangjuxu" (ST 37). Anti-neutrophil cytoplasmic antibody (ANCA) was detected with indirect immunofluorescence, and the pathological changes were investigated with pathological tissue section technique. Results The positive rate of ANCA reached to 62. 5 % in the rat with ulcerative colitis with significant differences among the 3 groups. After treatment, the positive rate of ANCA was decreased in the two treatment groups (P<0. 05, P<0. 01). Conclusion Acupoint embedding thread therapy has definite therapeutic effect on ulcerative colitis and the mechanism is possibly related with the decrease of ANCA.