德国小蠊DDVP抗性与相关酶活性的关系

目的 探讨乙酰胆碱酯梅（AChE）、α-乙酸萘酯酶（1-NA）、谷胱甘肽S-转移酶（GSTs）活性与德国小蠊敌敌畏（DDVP）抗性水平的关系。方法 采用分光光度法和微量板酶动力学法分别测定不同DDVP抗性水平德国小蠊的AChE、1-NA和GSTs酶活性，应用SAS软件进行统计分析。结果 随着德国小蠊DDVP抗性水平的增加，1-NA及GSTs酶活性逐渐性逐渐升高，AChE活性仰制率和1-NA酶活性在德国小蠊抗性系数为2．8及以下时与敏感株相比，差异均有统计学意义P〈0．05。GSTs酶活性在抗性系数2．8及以下与敏感株相比，差异无统计学意义。结论 AChE的抑制率和1-NA酶活性可作为德国小蠊对DDVP抗性的生物化学标记。

Relationship between DDVP resistance of German cockroaches and related enzyme

Objective To study the relationship between the activities of acetycholine esterase,1-NA,glutathione S-transferase and DDVP resistance of German cockroaches(Blattella germanica).Methods To measure the activities of enzymes above-mentioned in different DDVP-resistant stains of German cockroaches by using visible spectrophotometry and kinetic microplate methods,and to carry through the statistical analysis by SAS software.Results With DDvp-resistant levels' increased in Germand cockroaches,AChE's inhibition ratio,1-NA and glutathone S-transferase enhancd gradually.The distinction was remarkabkle on inhibition ratio of acetycholine esterase's activity and 1-NA's activity among resistant strains and sensitive strains when resistant coefficient equal or less than 2.8.Nevertheless,the distinction was not remarkable for the activity of glutathione s-transferase.Conclusion AChE's inhibition ratio and 1-NA's activity are proved to some values as biomarkers of German cockroaches'DDVP-resistance.