β-catenin与cyclinD1在胆道肿瘤中的表达及临床意义

目的通过观察胆道肿瘤组织中β-连环素（β-catenin）与细胞周期素D1（cyclinD1）的表达，探讨它们和胆道肿瘤发生、预后的关系。方法应用免疫组织化学法研究33例胆囊癌，35例胆管癌中β-catenin与cyclinD1的表达特点，并结合临床资料进行统计学分析。结果10例正常的胆囊和胆管标本中β-catenin与cyclinD1均呈正常表达。而在33例胆囊癌组织中有16例（48．5％）β-caterfin的异常表达和18例（54．5％）cyclinD1的过度表达；35例胆管癌组织中存在18例（51．4％）β-catenin的异常表达和16例（45，7％）cyclinD1的过度表达。在胆道恶性肿瘤中。β-catenin的表达异常和cyclinD1的过度表达与TNM分期和／或肿瘤的分化相关，而且β-catenin的异常表达与cyclinD1的过度表达相关（P〈0．05）。结论在胆道恶性肿瘤组织中存在β-catenin的异常表达与cyclinD1的过度表达。β-catenin的异常表达与cyclinD1的过度表达之间的相关性提示，β-catenin／Wnt信号转导途径参与了胆道肿瘤的发生和发展。

Expression of β-catenin and cyclinD1 in Biliary tumors and its clinical significance

Objective] To investigate expression of β-catenin and cyclinD1 and its possible role in the tumors' development and progression. Methods] Expression of β-catenin and cyclinD1 was immunohistochemically studied in gallbladder carcinoma (33 cases), and cholangiocarcinoma (35 cases), respectively. The results were analyzed with clinical-pathological data. Results] Normal expression of β-catenin and cyclinD1 was found in 10 cases of normal biliary control samples. Abnormal expression of β-catenin and cyclinD1 overexpression was detected in 48.5% (16/33) and 54.5% (18/33) in samples of gallbladder carcinoma respectively. Similarly, abnormal expression of β-catenin and cyclinD1 overexpression were also found in cholangiocarcinoma, 51.4% (18/35) and 45.7% (16/35) respectively.Abnormal expression of β-catenin and cyclinD1 overexpression were related to TNM stages and/or tumor differentiation in these biliary tumors. Furthermore, in both gallbladder carcinoma and cholangiocarcinoma, abnormal β-catenin expression was correlated with cyclinD1 overexpression (P ＜0.05). Conclusion] Abnormal expression of β-catenin and cyclinD1 overexpression exist in biliary tumors. Correlation between abnormal expression of β-catenin and cyclinD1 overexpression suggests that WNT/β-catenin signaling pathway plays a role in biliary tumors' development and progression.