Differences in protein synthesized in vivo and in vitro by cells associated with the cerebral microvasculature. A protein synthesized in response to trauma? |
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Authors: | F.P. White |
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Affiliation: | Faculty of Medicine, Memorial University of Newfoundland, St John''s, Newfoundland A1B 3V6, Canada |
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Abstract: | A protein of molecular weight 71,000 (P71) was synthesized by cells associated with the cerebral microvasculature in telencephalon slices incubated in vitro. This protein, however, was not synthesized in vivo. There was a 30 min delay in the synthesis of P71 by brain slices. During this lag period a new ribonucleic acid species which was required for P71 synthesis was produced in the slices. After this delay P71 became a major product of protein synthesis. P71 was also produced by microvasculature enriched fractions isolated from telencephalon.This protein may be involved in the initial response of the vascular system to injury. |
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Keywords: | BSA bovine serum albumin soluble fraction of the homogenate TCA trichloracetic acid |
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