| 中性胆固醇酯水解酶活性在COS细胞中高度表达 |
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| 引用本文： | 刘国庆,蔡海江,范乐明,陈秀英. 中性胆固醇酯水解酶活性在COS细胞中高度表达[J]. 南京医科大学学报(自然科学版), 1993, 0(4) |
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| 作者姓名： | 刘国庆 蔡海江 范乐明 陈秀英 |
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| 作者单位： | 美国加州大学洛杉矶分校分子生物学研究所(刘国庆)，南京医学院动脉粥样硬化研究中心(蔡海江，范乐明)，南京医学院动脉粥样硬化研究中心(陈秀英) |
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| 摘 要： | 将激素敏感酯酶(HSL)基因转染至COS细胞株后,使得HSL的mRNA和中性胆固醇酯酶(CEH)活性得到高度表达。CEH活性随重组质粒DNA量的增加而增高,对其进行了动力学分析,并对CEH活性的检测方法进行了探讨。
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| 关 键 词： | 中性胆固醇酯水解酶 COS细胞株 基因表达 |
High Expression of Neutral Cholesteryl Ester Hydrolase Activity in COS Cell |
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| Liu Guoqing,Cai Haijiang,Fan Lemin,Chen Xiuying University of California. High Expression of Neutral Cholesteryl Ester Hydrolase Activity in COS Cell[J]. Acta Universitatis Medicinalis Nanjing, 1993, 0(4) |
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| Authors: | Liu Guoqing Cai Haijiang Fan Lemin Chen Xiuying University of California |
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| Affiliation: | Liu Guoqing,Cai Haijiang,Fan Lemin,Chen Xiuying University of California,Institute of Molecular Biology,USA Atherosclerosis Research Center of Nanjing Medical College,Nanjing |
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| Abstract: | We introduced the gene of hormone sensitive lipase (HSL), which alsopossesses CEH activity, into a mammalian cell line, COS cell. Transfection by electropora-tion resulted in high expression of HSL mRNA and neutral CEH activity. The expression ofCEH activity was increased with the amount of construct DNA. The kinetics of expressedCEH was also analyzed at certain details and the method for CEH activity was optimized. |
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| Keywords: | neutral cholesteryl ester hydrolase COS cell line gene expression |
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